鲍勇刚, 朱馨蕾, 刘进, et al. 新疆家蚕抗菌肽(Cecropin-XJ)毕赤酵母高密度发酵条件的研究[J]. Journal of Xinjiang University (Natural Science Edition in Chinese and English), 2004, (3).
鲍勇刚, 朱馨蕾, 刘进, et al. 新疆家蚕抗菌肽(Cecropin-XJ)毕赤酵母高密度发酵条件的研究[J]. Journal of Xinjiang University (Natural Science Edition in Chinese and English), 2004, (3).DOI：
包括培养基中碳源、氮源、初始 p H值、磷酸盐等因素对毕赤酵母工程菌生长和抗菌肽分泌的影响 .通过多因素正交实验确定了生产新疆家蚕抗菌肽高密度发酵培养的最适培养条件
条件优化后毕赤酵母工程菌发酵后密度OD6 0 0 可达 48.92± 0 .5 9
较优化前提高了 3倍
为基因工程生产新疆家蚕抗菌肽奠定了基础
Abstract
The high-density culture conditions of a Pichia Pastoris strain prod ucing cecropin-XJ is studied
including the effect of various carbon sources
yeast powder
peptone
KH2PO4
and Initial pH. The optimum conditions are selected by orthogonal design. These results show the optimal density (OD600)of Pichia Pastoris strain containing the recombinant pGAPZαA-cecropin-XJ has achieved 48.92±0.59(OD600) in the optimum conditions
three times higher than in the original culture conditions.
CreggJM,T schoppJF,StillmanC,etal.High-level expression and efficientassembly of hepatitisB surface anti-gen in the methylo troph ic yeastPichia pastoris[J].BioTechnology,1987,5:479-485.
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