灰绿藜耐盐基因CgNHX转导新疆陆地棉的初步研究

徐栋生; 宁新民; 赵娟; 李秀明; 孔杰; 谷丽丽; 姚世响; 孔庆平; 兰海燕; 张富春

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灰绿藜耐盐基因CgNHX转导新疆陆地棉的初步研究

研究精粹 | 更新时间：2026-01-21

- 灰绿藜耐盐基因CgNHX转导新疆陆地棉的初步研究
- 灰绿藜耐盐基因CgNHX转导新疆陆地棉的初步研究
- 新疆大学学报（自然科学版中英文） 2009年26卷第1期页码：20-26
- 作者机构：
  
  1. 新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室
  2. 新疆农业科学院经济作物研究所
- 作者简介：
- 基金信息：
  
  新疆自治区十一五科技攻关重大专项项目(200731138-3);新疆生物资源与基因工程重点实验室开放基金(XJDX0201-2008-03)
- DOI：
  中图分类号： S562
- 纸质出版：2009
- 稿件说明：
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[1]徐栋生,宁新民,赵娟,等.灰绿藜耐盐基因CgNHX转导新疆陆地棉的初步研究[J].新疆大学学报(自然科学版),2009,26(01):20-26.

徐栋生, 宁新民, 赵娟, et al. 灰绿藜耐盐基因CgNHX转导新疆陆地棉的初步研究[J]. Journal of Xinjiang University (Natural Science Edition in Chinese and English), 2009, 26(1): 20-26.
[1]徐栋生,宁新民,赵娟,等.灰绿藜耐盐基因CgNHX转导新疆陆地棉的初步研究[J].新疆大学学报(自然科学版),2009,26(01):20-26. DOI：

徐栋生, 宁新民, 赵娟, et al. 灰绿藜耐盐基因CgNHX转导新疆陆地棉的初步研究[J]. Journal of Xinjiang University (Natural Science Edition in Chinese and English), 2009, 26(1): 20-26. DOI：

摘要

以新疆陆地棉为实验材料

通过花粉管通道法向优质棉花高代材料中导入了灰绿藜耐盐基因CgNHX.经过三年连续转导的结果显示:①大田注射期间的天气状况对转导成功影响较大.当昼夜温差较大且有露水时

花和铃的状态较好

导致结铃率、卡那霉素检测阳性率增加;②棉叶基因组DNA的提取质量与棉叶采后冷藏的时间有一定相关性.采后一周内提取的棉叶DNA质量较好

PCR检测获得30%的阳性率

而两周及以后的棉叶DNA质量降低

PCR检测未获得阳性结果;③在mRNA水平上检测转基因植株的表达较为困难.本实验中PCR检测阳性的30份样品

在RT-PCR检测中均未获得目的基因的明显特异扩增条带

只有部分样品在目的条带的位置出现弥散带型.本研究初步显示外源基因CgNHX已导入棉花受体中

但其表达情况还需进一步检测验证.

Abstract

By Using Xinjiang Upland cotton as experimental materials

the salt-tolerant gene CgNHX from Chenopodium glaucum was introduced into the high-quality cotton lines through the pollen-tube pathway.After 3 years’DNA injection

results showed that:①The weather condition within the injection period in field had great effect on the success of transduction.With the larger temperature difference between day and night

and with some dew

the flower and boll would be in a good developmental condition

and which resulted in a higher boll-setting and kanamycin-resistant rate;②The quality of genomic DNA of cotton leaf was co-related with the time duration of low-temperature storage of leaf after sampling.DNA extraction within one week of post-harvest had higher quality and the PCR-positive rate was 30%

while the DNA quality was much poor and with no PCR-positive sample when extraction in more than one week;③It was diffcult to detect the expression of transgenic plants at mRNA level.With 30 PCR-positive samples we got in this study

there had no sample with obviously specific amplification band of target gene in RT-PCR test

only some samples showed diffusion bands at the right position.This study preliminarily showed that the foreign gene CgNHX have been introduced into cotton material

but the expression of CgNHX still need to be verified with further test.

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