新疆妇女宫颈癌组织中HPV-16E1/dup突变体分布的研究

侯向前; 史茜; 朱开春; 玛依努尔·尼亚孜; 马正海

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新疆妇女宫颈癌组织中HPV-16E1/dup突变体分布的研究

研究精粹 | 更新时间：2026-01-21

- 新疆妇女宫颈癌组织中HPV-16E1/dup突变体分布的研究
- 新疆妇女宫颈癌组织中HPV-16E1/dup突变体分布的研究
- 新疆大学学报（自然科学版中英文） 2012年29卷第4期页码：384-389
- 作者机构：
  
  1. 新疆大学生命科学与技术学院
  2. 新疆自治区人民医院
- 作者简介：
- 基金信息：
  
  国家自然科学基金(31060025);新疆生物资源基因工程重点实验室开放课题
- DOI：
  中图分类号： R737.33
- 纸质出版：2012
- 稿件说明：
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[1]侯向前,史茜,朱开春,等.新疆妇女宫颈癌组织中HPV-16 E1/dup突变体分布的研究[J].新疆大学学报(自然科学版),2012,29(04):384-389+505.

侯向前, 史茜, 朱开春, et al. 新疆妇女宫颈癌组织中HPV-16E1/dup突变体分布的研究[J]. Journal of Xinjiang University (Natural Science Edition in Chinese and English), 2012, 29(4): 384-389.
[1]侯向前,史茜,朱开春,等.新疆妇女宫颈癌组织中HPV-16 E1/dup突变体分布的研究[J].新疆大学学报(自然科学版),2012,29(04):384-389+505. DOI：

侯向前, 史茜, 朱开春, et al. 新疆妇女宫颈癌组织中HPV-16E1/dup突变体分布的研究[J]. Journal of Xinjiang University (Natural Science Edition in Chinese and English), 2012, 29(4): 384-389. DOI：

摘要

目的检测HPV-16 1311～1773位63bp重复序列插入突变的E1基因(E1/dup)在宫颈炎、宫颈上皮内瘤变(cervical intraepithelial neoplasia

CIN)及宫颈癌病变组织中的分布及病毒的整合状态

探讨该突变对病毒致癌活性的影响.方法收集维吾尔族和汉族宫颈炎、CIN和宫颈癌临床组织标本215例

提取组织DNA

PCR检测E1/dup突变体以及病毒E1/E2基因的完整性.结果新疆地区215例宫颈病变组织中HPV-16 E1检出率为60.00%(129/215)

宫颈炎、CIN及宫颈癌病样中HPV-16 E1的检出率分别为31.15%(19/61)、49.21%(31/63)和86.81%(79/91).在HPV-16E1阳性病样中

HPV-16 E1/dup突变率为37.21%(48/129)

宫颈炎、CIN和宫颈癌病样中E1/dup突变率分别为15.79%、22.58%和48.10%

E1/dup突变率在宫颈癌中显著高于宫颈炎(P<0.05)和CIN(P<0.05).E1/dup突变率在汉族和维吾尔族宫颈癌病样中分别为40.00%(6/15)和50.00%(32/64)

两者无显著性差异(P>0.05).在HPV-16 E1阳性病样中病毒整合率为51.72%(60/116)

E1/dup突变病样中HPV16整合率[69.05%(29/42)]明显高于未发生E1/dup突变的病样[41.89%(31/74)](P<0.05).结论新疆地区E1/dup突变率在宫颈癌中显著高于宫颈炎及CIN

E1/dup与病毒致癌活性及病毒整合相关.

Abstract

Objective To detect the distribution of HPV-16 E1 gene mutant with the insertion of 63bp nucleotide duplication sequences at position 1311-1373(E1/dup) from clinical tissue specimens of cervicitis

cervical intraepithelial neoplasia(CIN) and cervical cancer

and define the integrated status of HPV-16

then research into the carciongenic potency of E1/dup.Methods 215 clinical tissue specimens of cervicitis

CIN and cervical cancer from Uygur and Han patients were collected

the tissue DNAs were extracted.Then E1/dup mutant and integral E1/E2 were detected by PCR.Results The positive rate of HPV-16 E1 in the 215 specimens was 60.00%(129/215)

and the rates in cervicitis

CIN and cervical cancer were 31.15%(19/61)

49.21%(31/63) and 86.81%(79/91) respectively.In the HPV-16 E1 positive specimens

the rates of E1/dup mutant were 37.21%(48/129)

and the rate of E1/dup mutant from the cervical cancer(48.10%) was significantly higher than that from the cervicitis(15.79%)(P<0.05) and CIN(22.58%)(P<0.05).The rates of E1/dup mutant from Han cervical cancer and Uygur cervical cancer specimens were 40.00%(6/15) and 50.00%(32/64) respectively

there were no significant differences between of them(P>0.05).The rate of integrated HPV-16 from the E1 positive specimens was 51.72%(60/119)

and the rate[69.05%(29/42)] from the E1/dup Specimens was significantly higher than the rate [41.89%(31/74)] from no-E1/dup Specimens.Conclusion The rate of E1/dup mutant from the cervical cancer was significantly higher than that from the cervicitis and CIN

and E1/dup mutant was associated with the carciongenic potency and integrated status of HPV-16.

关键词

Keywords

references

拉莱.苏祖克.不同民族妇女中2727例子宫颈癌的统计学分析[J].新疆医学院学报,1990,9:48.

Jemal A,Bray F,Center MM,et al.Global cancer statistics[J].CA Cancer J Clin,2011,61(2):69-90.

Jonathan R C,Ding Z Q,Barbara R.HPV infection and cervical disease:A review[J].Australian and New Zealand Journalof Obstetrics and Gynaecology,2011,51(2):103-108.

Zur Hausen H.Human papillomavirus in the pathogenesis of anogenital cancer[J].Virology,1991,184(1):9-14.

Romanczuk H,Howley P M.Disruption of either the E1 or E2 regulatory gene of human papillomavirus type 16 increasesviral immortalization capacity[J].Proc Natl Acad Sci USA,1992,89(7):3159-3163.

董小平,周伟,Pfister H.染色体外游离态的子宫颈癌HPV16分离株E1/E2区的基因序列重排[J].中华实验和临床病毒学杂志,2000,14(1):5-8.

Yasugi T,Benson J D,Sakai H,et al.Mapping and Characterization of the Interaction Domains of Human PapillomavirusType 16 E1 and E2 Proteins[J].J Virol,1997,71(2):891-899.

许璐,刘立鸿,李轶杰,等.新疆株HPV-16L1在真核细胞中表达水平的初探[J].细胞生物学杂志,2008,30(4):499-503.

马正海,钱东,马纪,等.中国新疆维吾尔族妇女宫颈癌组织中乳头状瘤病毒16型E6基因的克隆和序列分析[J].生物化学与生物物理进展,2001,28(3):400-404.

马正海,张富春,梅新娣,等.新疆南部地区维吾尔族妇女宫颈癌组织中人乳头状瘤病毒16型L1基因突变谱分析[J].中华医学杂志,2004,84(12):987-991.

马正海,梅新娣,张富春.新疆南部地区维吾尔族妇女宫颈癌组织中HPV-16型L2基因多态性突变谱分析[J].中华微生物学和免疫学杂志,2004,24(12):968-972.

余勐,马正海,王艳萍,等.新疆维吾尔族妇女宫颈癌组织中人乳头状瘤16型上游调控区DNA多态性分析[J].中华微生物学和免疫学杂志,2006,26(11):1000-1004.

Ivan Sabol,Mihaela Matovina,Nina Milutin Gasperov,et al.Identification of a novel human papillomavirus type 16 E1 genevariant with potentially reduced oncogenicity[J].Journal of Medical Virology,2008,80(12):2134-2140.

Ivan Sabol,Mihaela Matovina,Ali Si-Mohamed,et al.Characterization and Whole Genome Analysis of Human PapillomavirusType 16 E1-1374∧63nt Variants[J].PLoS One,2012,7(7):e41045.

Bogovac Z,Lunar MM,Kocjan BJ,et al.Prevalence of HPV 16 genomic variant carrying a 63-bp duplicated sequence withinthe E1 gene in Slovenian women[J].Acta Dermatovenerol Alp Panonica Adriat,2011,20(3):135-139.

Kalantari M,Blennow E,Hagmar B,et al.Physical state of HPV-16 and chromosomal mapping of the integrated form incervical carcinomas[J].Diagn Mol Pathol,2001,10:46-54.

Pirami L,Giache V,Becciolini A.Analysis of HPV-16,18,31,and 35 DNA in pre-invasive and invasive lesions of the uterinecervix[J].J Clin Pathol,1997,50:600-604.

Park JS,Hwang ES,Park SN,et al.Physical status and expression of HPV genes in cervical cancers[J].Gynecol Oncol,1997,65:121-129.

Daniel B,Mukherjee G,Seshadri L,et al.Changes in the physical state and expression of human papillomavirus type 16 inthe progression of cervical intraepithelial neoplasia lesions analysed by PCR[J].J Gen Virol,1995,76:2589-2593.

Badaracco G,Venuti A,Sedati A,et al.HPV-16 and HPV18 in genital tumors:significantly different levels of viral integrationand correlation to tumor invasiveness[J].J Med Virol,2002,67:574-582.

Arias-Pulido H,Peyton CL,Joste NE,et al.Human papillomavirus type 16 integration in cervical carcinoma in situ and ininvasive cervical cancer[J].J Clin Microbio,2006,l44:1755-1762.

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