1. 新疆大学生命科学与技术学院
2. 齐齐哈尔大学生命科学与农林学院
纸质出版:2013
移动端阅览
[1]买买提明·苏来曼,徐红红,玛尔孜亚·阿不力米提,等.水藓科植物遗传多样性研究[J].新疆大学学报(自然科学版),2013,30(04):390-395.
买买提明·苏来曼, 徐红红, 玛尔孜亚·阿不力米提, et al. 水藓科植物遗传多样性研究[J]. Journal of Xinjiang University (Natural Science Edition in Chinese and English), 2013, 30(4): 390-395.
利用ISSR分子标记方法对不同地区的13份水藓科植物进行遗传多样性分析
结果表明
稳定且重复性高的7条引物共扩增出526条清晰的条带
其中518条具有多态性
平均多态性位点比率为98.47%.运用PopGen-32软件分析发现
等位基因数Na=1.736 3
有效等位基因数Ne=1.306 2
Nei基因的多样性与Shannon信息的指数分别为0.201 5和0.321 1
说明水藓科植物的遗传多样性比较丰富.13份水藓科植物的遗传分化系数Gst、遗传距离D与遗传一致度I分别为0.025 3、0.007 1和0.054 7
对其进行计算得知
不同属种间存有基因交流
但与种间变异相比
种群内部的遗传变异较大.通过NTSYS2.1软件对13份样本的亲缘关系进行聚类
可将其分为3类
且样本的遗传距离与地理位置有一定的相关性.
The genetic diversity of 13 species Fontinalaceae collected in diferent regions was studied using ISSR marker technique method. The results showed that 526 clear bands were amplified by 7 stable and highly reproducible primers of which 518 were polymorphic. the percentage of polymorphic bands reached 98.47%. The result of analyzed by the software POPGENE-32 showed that number of alleles Na=1.7363
efective number of alleles Ne=1.3062. Nei gene diversity and Shannon information index were 0.201 5 and 0.321 1. It showed that the geographical distribution was very obvious. The coefcient of gene diferentiation Gst
genetic distance D and genetic identity I of 13 samples were 0.025 3
0.007 1 and 0.054 7
indicating there was a certain gene flow among diferent species of Fontinalaceae and the genetic variation was mainly within species compared with interspecies. Cluster analysis among 13 samples genetic relationship using software NTSYS2.1 showed that these samples were divided into there groups. In addition
there were some correlation between genetic distance and geographic distance among the samples.
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