铝胁迫及酸度对天山雪岭云杉菌根真菌生长的影响

王艳阳; 常顺利; 王诗慧; 古丽哈娜提·波拉提别克; 张毓涛

doi:10.13568/j.cnki.651094.651316.2019.03.14.0001

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铝胁迫及酸度对天山雪岭云杉菌根真菌生长的影响

生命科学与技术 | 更新时间：2026-01-19

- 铝胁迫及酸度对天山雪岭云杉菌根真菌生长的影响
- 铝胁迫及酸度对天山雪岭云杉菌根真菌生长的影响
- 新疆大学学报（自然科学版中英文） 2020年37卷第1期页码：55-62
- 作者机构：
  
  1. 新疆大学资源与环境科学学院绿洲生态教育部重点实验室
  2. 新疆林业科学院森林生态研究所
- 作者简介：
- 基金信息：
  
  国家自然科学基金(U1503187);“十二五”农村领域国家科技计划(2015BAD07B03-03)
- DOI：10.13568/j.cnki.651094.651316.2019.03.14.0001
  中图分类号： S791.18
- 纸质出版：2020
- 稿件说明：
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[1]王艳阳,常顺利,王诗慧,等.铝胁迫及酸度对天山雪岭云杉菌根真菌生长的影响[J].新疆大学学报(自然科学版)(中英文),2020,37(01):55-62.
[1]王艳阳,常顺利,王诗慧,等.铝胁迫及酸度对天山雪岭云杉菌根真菌生长的影响[J].新疆大学学报(自然科学版)(中英文),2020,37(01):55-62. DOI： 10.13568/j.cnki.651094.651316.2019.03.14.0001.

DOI：10.13568/j.cnki.651094.651316.2019.03.14.0001.

摘要

天山雪岭云杉(Picea schrenkiana)森林林下土壤呈酸性

酸性条件(pH<5.5)易引起土壤中活性铝浓度的增加

进而抑制菌根真菌和植物的生长.本文以从天山雪岭云杉根部分离得到的拟青霉(Simplicillium sp.

Si)、细长孢霉(Mortierella elongate

Me)、木霉菌(Trichoderma spp.

Tr)、瓶头霉(Phialocephala

Ph)和葡萄状穗霉(Stachybotrys chartarum

Sc)5种共生菌根真菌为供试材料

在p H值条件为6.5和5.5下设定6个Al3+浓度梯度(0、0.2、0.4、0.6、0.8、1.0mmol·L-1)

另单独设置5个p H值条件(4.5、5.0、5.5、6.0、6.5)

利用PDA固体培养基培养菌株

测定其生长指标

比较活性铝浓度和p H值对菌根真菌生长的影响.结果表明:(1)高铝(1.0 mmol·L-1)处理后

仅Tr菌落直径比对照增加了11.4%

Me和Ph受活性铝影响不明显

Si和Sc菌落直径显著低于对照(P <0.05);(2)低pH值(4.5)未对Tr和Me菌落直径产生显著影响

却对Ph、Si和Sc产生抑制作用

显著低于对照(P <0.05);(3)5种菌株在高铝和低pH值条件下表现出不同的抗酸铝特性.在低pH值和高铝影响下

Tr菌落直径达到最大

Ph和Me菌落直径分别比对照增加了14.1%和2.3%

而Si和Sc分别比对照减少了5.0%和5.3%.因此

Tr、Me和Ph具有较强的抗铝能力

属耐铝型

Si和Sc具有较差的抗铝能力

属铝敏感型.Tr于酸铝胁迫下生长速度最快

菌落直径最大

应是能快速缓解铝毒的最具潜力菌株.

Abstract

The Picea spruce forest of Tianshan Mountains soil is acidic(pH<5.5)

which causes the concentration of active Aluminium in soil increased easily

and the growth of mycorrhizal fungi and plants is inhibited. In this paper

?ve mycorrhizal fungi of Simplicillium sp. Si

Mortierella elongate Me

Trichoderma spp. Tr

Phialocephala Ph and Stachybotrys chartarum Sc isolated from the roots of Picea schrenkiana in Tianshan Mountains were used as test materials

and six Al3+ concentration gradients(0

0.2

0.4

0.6

0.8

1.0 mmol·L-1) and ?ve pH conditions(4.5

5.0

5.5

6.0

6.5) were separately set. The strains were cultured in PDA solid medium to measure their growth indexes

and the effects of active Aluminum concentration and pH on the growth of mycorrhizal fungi were analyzed. The results showed that:(1) After the high concentration of Aluminum(1.0 mmol·L-1) treatment

only Tr colony diameter increased by 11.4% compared to the control

Me and Ph were not signi?cantly affected by Aluminum

colony diameter of Si and Sc was signi?cantly lower than the control(P <0.05);(2) Low pH value did not signi?cantly affect Tr and Me colony diameter

but inhibited Ph

Si and Sc growth signi?cantly compared to that of the control(P <0.05);(3) Aluminum resistant property of ?ve strains exhibited differences at high Aluminum and low p H. The diameter of Tr colony reached the maximum

Ph and Me colony increased by 14.1%and 2.3%

respectively

while Si and Sc decreased by 5.0% and 5.3%

respectively. In conclusion

Me and Ph are Aluminium resistance type

while Si and Sc are Aluminium sensitive type. Tr has the fastest growth rate and the largest colony diameter under the acid and Aluminium stress

which is the most potential strain can quickly alleviate Aluminium toxicity.

关键词

Keywords

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