新疆乌苏天山山地南坡草原革蜱中立克次体感染调查及遗传进化分析

孙素荣; 王思远; 史深; 张渝疆; 张敬媛; 袁逸伦; 杨小飞; 张媛; 丁焱辉

doi:10.13568/j.cnki.651094.651316.2019.09.24.0003

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新疆乌苏天山山地南坡草原革蜱中立克次体感染调查及遗传进化分析

生命科学与技术 | 更新时间：2026-01-21

- 新疆乌苏天山山地南坡草原革蜱中立克次体感染调查及遗传进化分析
- 新疆乌苏天山山地南坡草原革蜱中立克次体感染调查及遗传进化分析
- 新疆大学学报（自然科学版中英文） 2020年37卷第2期页码：190-196
- 作者机构：
  
  1. 新疆大学生命科学与技术学院新疆生物资源与基因工程重点实验室
  2. 新疆维吾尔自治区疾病预防控制中心
- 作者简介：
- 基金信息：
  
  国家自然科学基金（81760365;81690369）;新疆高校科研计划自然科学重点项目（XJEDU2019I002）
- DOI：10.13568/j.cnki.651094.651316.2019.09.24.0003
  中图分类号： S855
- 纸质出版：2020
- 稿件说明：
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[1]孙素荣,王思远,史深,等.新疆乌苏天山山地南坡草原革蜱中立克次体感染调查及遗传进化分析[J].新疆大学学报(自然科学版)(中英文),2020,37(02):190-196.
[1]孙素荣,王思远,史深,等.新疆乌苏天山山地南坡草原革蜱中立克次体感染调查及遗传进化分析[J].新疆大学学报(自然科学版)(中英文),2020,37(02):190-196. DOI： 10.13568/j.cnki.651094.651316.2019.09.24.0003.

DOI：10.13568/j.cnki.651094.651316.2019.09.24.0003.

摘要

了解新疆乌苏地区草原革蜱中立克次体的感染情况.采集新疆乌苏天山山地南坡的草原革蜱

通过PCR对蜱中斑点热群立克次体、贝氏柯克斯体、埃立克体、伯氏疏螺旋体、查菲埃立克体、无形体、嗜吞噬细胞无形体、牛巴贝斯虫、巴贝西原虫、利什曼原虫、恙虫立克次氏体等11种病原体进行DNA分子检测

对检出的阳性样本进行测序

并与GenBank (NCBI)中的相应基因序列进行比对分析.106只草原革蜱中斑点热群立克次体、贝氏柯克斯体、埃立克体检测结果为阳性

阳性率分别为57.23%(99/173)、37.50%(30/80)和37.74%(20/53)

其余8种病原体检测结果均为阴性;对斑点热群立克次体进行序列分析

建立分子系统进化树

结果显示Guertu-Dn1801 OmpA与吉林两株M198 (MF511260.1)和M30 (MF511254.1)同源性最高

一致性均为96.90%

属劳氏立克次体;Guertu-Dn1803OmpA与俄罗斯Gorno-Altaisk-2014-H-2株(KT006594.1)和苏联246株(U43807.1)同源性最高

一致性均为94.40%

属西伯利亚立克次体;而Guertu-Dn1801 gltA和Guertu-Dn1809 gltA分别与黑龙江3LB01 (KM245567.1)和内蒙古MD0202 (KP742992.1)序列一致性均为100%

属劳氏立克次体.乌苏地区可能存在斑点热群立克次体、贝氏柯克斯体、埃立克体3种病原体的流行

且斑点热群立克次体感染率较高

应加强对当地蜱类和蜱传疾病的监测和防控.

Abstract

To investigate the infection of Rickettsia in Dermacentor nuttalli of the southern slope of the Tianshan Mountain of Wusu

Xinjiang.Dermacctor nuttalli were collected in Wusu region of Xinjiang

PCR was used to detect the DNA of 11 pathogens such as spotted fever group Rickettsia

Coxiella burnetii

Ehrlichia

Borrelia

Ehrlichia chaffeensis

Anaplasma Anaplasma-phagocytophilum Babesia

Babesia spp.

Leishmani-a

Oresteia tsutsugamushi in ticks.The positive samples were sequenced and compared with the corresponding gene sequences deposited in GenBank(NCBI).Among 106 of Dermacentor nuttalli

the positive rates of infection spotted fever group Rickettsia

Coxiella burnetii and Ehrlichia were 57.23%(99/173)

37.50%(30/80) and 37.74%(20/53)

respectively.The other eight pathogens were all negative.The sequence analysis of spotted fever group Rickettsia was carried out and the phylogenetic tree was established.The results showed that Guertu-Dn1801 OmpA had the highest homology with Jilin province two strains M198(MF511260.1) and M30(MF511254.1) both of which were 96.9%homology

belonging to Rickettsia raoultii.Compared with Russia strain Gorno-Altaisk-2014-H-2(KT006594.1)and Soviet Union strain 246(U43807.1)

Guertu-Dn1803 OmpA had 94.4% homology with them and belonged to Rickettsia sibirica.Guertu-Dn1801 gltA and Guertu-Dn1809 gltA were on the same branch with Heilongjiang province strain 3 LB01(KM245567.1) and Inner Mongolia strain MD0202(KP742992.1) respectively

and the identity was 100%

belonged to Rickettsia raoultii.There may be epidemics of spotted fever group Rickettsia

Coxiella burnetii and Ehrlich in Wusu region

and the carrying rate of spotted fever group Rickettsiae was high.Monitoring and controlling of local tick and tickborne diseases should be strengthened.

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references

CHEN Z,LIU Q,LIU J Q,et al.Tick-borne pathogens and associated co-infections in ticks collected from domestic animals in central China[J].Parasit Vectors,2014,7(1):237-244.

于心,叶瑞玉.新疆蜱类与疾病关系的研究[J].疾病预防控制通报,1996(4):46-47.YU X,YE R Y.A study on the relationship between ticks and infectious diseases in Xinjiang[J].Bulletin of Disease Control and Prevention,1996(4):46-47.(in Chinese)

WANG Y Z,MU L M,ZHANG K,et al.A broad-range survey of ticks from livestock in Northern Xinjiang:changes in tick distribution and the isolation of Borrelia burgdorferi sensu strict[J].Parasites&Vectors,2015,8(1):1-9.

YIN H,LU W,LUO J.Babesiosis in China[J].Trop Anim Health Prod,1997,29(4s):11-15.

KULAKOVA N V,KHASNATINOV M A,SIDOROVA E A,et al.Molecular identification and phylogeny of Dermacentor nutalli(Acari:Ixodidae)[J].Parasitology Research,2014,113(5):1787-1793.

SHEN S,DUAN X M,WANG B,et al.A novel tick-borne phlebovirus,closely related to severe fever with thrombocytopenia syndrome virus and heartland virus,might be a potential pathogen[J].Emerging Microbes&Infections,2018,7(1):1-14.

袁江铃,马素贞,沈炯玉,等.牛巴贝斯虫新疆株MSA-2c基因重组质粒的构建真核表达及其免疫反应性研究[J].新疆农业大学学报,2008,31(4):79-82.YUAN J L,MA S Z,SHEN J Y,et al.Construction,expression and immunological reactivity of Xinjiang Babesia bovis MSA-2c gene recombination plasmid[J].Journal of Xinjiang Agricultural University,2008,31(4):79-82.(in Chinese)

崔红,张雪颖,宫占威,等.巢式聚合酶链反应快速检测贝氏柯克斯体的研究[J].中华检验医学杂志,2000,23(2):74-76.CUI H,ZHANG X Y,GONG Z W,et al.Nested PCR for rapid detection of Coxiella burnetiid[J].Chinese Journal of Laboratory Medicine,2000,23(2):74-76.(in Chinese)

冯立,王卓,杨军,等.黑龙江逊克地区森林革蜱斑点热立克次体DNA的检测[J].中国人兽共患病学报,2014,30(10):1020-1023.FENG L,WANG Z,YANG J,et al.Molecular detection of spotted fever group rickettsia in Dermacentor silvarum form the Xunke Area,China[J].Chinese Journal of Zoonoses,2014,30(10):1020-1023.(in Chinese)

赵庆亮,黄林,杨霞,等.新疆部分地区鼠类无形体和埃立克体的调查及16s rRNA序列分析[J].中国人兽共患病学报,2013,29(8):743-747.ZHAO Q L,HUANG L,YANG X,et al.16s rRNA sequence analysis of Anaplasma and Ehrlichia in rodents from parts area of Xinjiang[J].Chinese Journal of Zoonoses,2013,29(8):743-747.(in Chinese)

孙响,张桂林,刘晓明,等.新疆和硕地区主要蜱类及蜱媒病原体调查[J]中国媒介生物学及控制杂志,2013,24(1):5-7.SUN X,ZHANG G L,LIU X M,et al.Investigation of tick species and tickborne pathogens in Hoxud county of Xinjiang Uyghur Autonomous Region,China[J].Chinese Journal of Vector Biology and Control,2013,24(1):5-7.(in Chinese)

张桂林,孙响,赵焱,等.新疆和硕地区荒漠景观中无形体感染的流行病学调查[J].中华流行病学杂,2013,34(2):147-151.ZHANG G L,SUN X,ZHAO Y,et al.Prevalence of Anaplasma spp.infection in a desert landscape region of Heshuo,Xinjiang[J].Chinese Journal of Epidemiology,2013,34(2):147-151.(in Chinese)

屈金辉,王洪法,赵桂华,等.新疆喀什地区利什曼原虫人群感染率调查[J].中国热带医学,2010,10(11):1324-1327.QU J H,WANG H F,ZHAO G H,et al.Survey of Leishmani donivani infection in humans in Kashi city of Xinjiang Uggur Autonomous Region[J].China Tropical Medicine,2010,10(11):1324-1327.(in Chinese)

郭恒彬,吴光华,潘秀珍,等.用PCR检测现场单个小盾纤恙螨体内恙虫病立克次体的研究[J].中国人兽共患病学报,1996,12(1):10-11.GUO H B,WU G H,PAN X Z,et al.Studies on detection of Rickettsia tsutsugamushi DNA in the single larva of Leptotrombidum(L.)scutellare collected from the endemic areas by PCR[J].Chinese Journal of Zoonoses,1996,12(1):10-11.(in Chinese)

张倩,刘运喜,吴晓明,等.中国内蒙古、新疆部分地区鼠类自然感染恙虫病东方体的调查[J].中华流行病学杂志,2006,27(6):475-478.ZHANG Q,LIU Y X,WU X M,et al.Investugation on rodents'natural infection of Orientia tsutsugamushi in some areas of Inner Mongolia and Xinjiang,China[J].Chinese Journal of Epidemiology,2006,27(6):475-478.(in Chinese)

姜理平,张磊,鲍庆汉,等.浙江省在鼠类中检测到巴贝西原虫DNA片段[J].中国媒介生物学及控制杂志,2012,23(4):303-305.JIANG L P,ZHANG L,BAO Q H,et al.Babesia DNA segments detected from rodents in Zhejiang province[J].Chinese Journal of Vector Biology and Control,2012,23(4):303-305.(in Chinese)

LIU L J,CHEN Q,YANG Y,et al.Investigations on Rickettsia in ticks at the Sino-Russian and Sino-Mongolian borders,China[J].Vector Borne Zoonotic Dis,2015,15(12):785-789.

FAN M Y,WANG J G,JIANG Y X,et al.Isolation of a spotted fever group rickettsia from a patient and related ecologic investigations in Xinjiang Uygur Autonomous Region of China[J].Journal of Clinical Microbiology,1987,25(4):628-632.

ROUX V,FOURNIER P E,RAOULT D.Differentiation of spotted fever group rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCR-amplified DNA of the gene encoding the protein rOmpA[J].Journal of Clinical Microbiology,1996,34(9):2058-2065.

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