新疆一枝蒿醇提物未除多糖与除多糖对DCs成熟的影响差异

张爱莲; 杨雨

doi:10.13568/j.cnki.651094.651316.2020.02.20.0002

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新疆一枝蒿醇提物未除多糖与除多糖对DCs成熟的影响差异

生命科学与技术 | 更新时间：2026-01-21

- 新疆一枝蒿醇提物未除多糖与除多糖对DCs成熟的影响差异
- 新疆一枝蒿醇提物未除多糖与除多糖对DCs成熟的影响差异
- 新疆大学学报（自然科学版中英文） 2020年37卷第4期页码：508-514
- 作者机构：
  
  新疆大学生命科学与技术学院新疆生物资源与基因工程重点实验室
- 作者简介：
- 基金信息：
  
  国家自然科学基金(31360224;31660259)
- DOI：10.13568/j.cnki.651094.651316.2020.02.20.0002
  中图分类号： R285
- 纸质出版：2020
- 稿件说明：
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[1]张爱莲,杨雨.新疆一枝蒿醇提物未除多糖与除多糖对DCs成熟的影响差异[J].新疆大学学报(自然科学版)(中英文),2020,37(04):508-514.
[1]张爱莲,杨雨.新疆一枝蒿醇提物未除多糖与除多糖对DCs成熟的影响差异[J].新疆大学学报(自然科学版)(中英文),2020,37(04):508-514. DOI： 10.13568/j.cnki.651094.651316.2020.02.20.0002.

DOI：10.13568/j.cnki.651094.651316.2020.02.20.0002.

摘要

比较新疆一枝蒿未除多糖醇提物(Ethanol extract of wild Artemisia rupestris L.

EEWAR)和除多糖醇提物(Ethanol extract of wild Artemisia rupestris L removing polysaccharides

DEEWAR)的免疫调节活性.利用体外树突状细胞(Dendritic cells

DCs)为平台

FACS (Fluorescence Activating Cell Sorter)技术检测未除多糖EEWAR和除多糖EEWAR对DCs成熟的影响.超声法提取EEWAR

检测其多糖和黄酮含量.C57BL/6小鼠骨髓细胞进行体外培养

第7 d后收获DCs

不同剂量未除多糖及除多糖的EEWAR分别刺激DCs细胞12 h后

LPS为阳性对照

检测DCs表面分子(CD11c

CD40

CD80

CD86和MHCII)表达情况.未除多糖和除多糖的EEWAR多糖含量分别为30.1%

5.3%

黄酮含量分别为5.6%

16.4%.FACS实验结果显示

未除多糖及除多糖的EEWAR均能够剂量依赖性促进CD80

CD86和MHCII分子的表达水平

且除多糖后促进DCs成熟的能力增强了约一倍;未除多糖及除多糖的EEWAR对CD40分子的促进作用不明显.结果表明

EEWAR除多糖后免疫调节活性明显增强.

Abstract

To compare the immunoregulatory activities of ethanol extract of wild Artemisia rupestris L.

(EEWAR)and ethanol extract of wild Artemisia rupestris L.(DEEWAR) removing polysaccharides. Using Dendritic cells(Dendritic cells

DCs) as a platform in vitro

The effects of EEWAR with polysaccharide or polysaccharide removal on DCs maturtion were detected by Fluorescence Activating Cell Sorter(FACS). EEWAR was extracted by ultrasonic method and its content of sugar and flavonoid was detected. Marrow-derived cells from C57BL/6 mice were cultured in vitro

and DCs were harvested on day 7 after culture. DCs were stimulated for 12 h by EEWAR with different doses

respectively. Expression of DCs surface molecules(CD11c

CD40

CD80

CD86 and MHCII)were detected. The content of polysaccharides and flavonoid of EEWAR was 30.1%

5.3%

5.6% and 16.4%

respectively. FACS results showed that EEWAR with polysaccharide or polysaccharide removal could increase the expression levels of CD80

CD86 and MHCII molecules in a dose-dependent manner

and the ability to promote DCs maturation after removing polysaccharides was doubled. The effect of EEWAR on CD40 was not obvious.The results showed that the immunomodulatory activity of EEWAR was significantly enhanced after the removal of polysaccharides.

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