石油污染土壤自然老化过程中微生物菌群及功能基因变化规律

李兴海; 吕杰; 马媛; 吕光辉

doi:10.13568/j.cnki.651094.651316.2020.03.02.0001

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石油污染土壤自然老化过程中微生物菌群及功能基因变化规律

生态·资源·环境 | 更新时间：2026-01-21

- 石油污染土壤自然老化过程中微生物菌群及功能基因变化规律
- 石油污染土壤自然老化过程中微生物菌群及功能基因变化规律
- 新疆大学学报（自然科学版中英文） 2021年38卷第4期页码：472-480
- 作者机构：
  
  新疆大学资源与环境科学学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(31860149;31360131);新疆大学大学生创新训练计划项目(201510755170)
- DOI：10.13568/j.cnki.651094.651316.2020.03.02.0001
  中图分类号： S154.3;X53
- 纸质出版：2021
- 稿件说明：
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[1]李兴海,吕杰,马媛,等.石油污染土壤自然老化过程中微生物菌群及功能基因变化规律[J].新疆大学学报(自然科学版)(中英文),2021,38(04):472-480.
[1]李兴海,吕杰,马媛,等.石油污染土壤自然老化过程中微生物菌群及功能基因变化规律[J].新疆大学学报(自然科学版)(中英文),2021,38(04):472-480. DOI： 10.13568/j.cnki.651094.651316.2020.03.02.0001.

DOI：10.13568/j.cnki.651094.651316.2020.03.02.0001.

摘要

本文旨在揭示新疆准东油田石油污染土壤自然老化过程中微生物菌群结构及芳香烃双加氧酶基因多态性及其变化规律.采集同一采油区3个不同污染年限土壤样本及背景土

进行理化指标检测、16S rDNA V4-V5区扩增子测序及芳香烃双加氧酶基因克隆文库构建与分析.扩增子测序分析结果显示

采油区不同背景土壤中原核微生物种类具有极大的相似性

并且污染1年的土壤中微生物种类与背景土微生物种类相似性较大;污染3年和5年样本间有一定差异

组内差异较小.克隆文库共获得46个芳香烃双加氧酶基因OTUs(Operational Taxonomic Units)

其Genbank注册号为KY985598-KY985643

分为邻苯二甲酸双加氧酶基因、非培养放线菌芳香加氧酶基因、萘双加氧酶基因、多环芳烃双加氧酶基因和苯甲酸双加氧酶基因5个基因类型

不同石油污染年限的土壤中双加氧酶基因类型会有显著性差异.对不同污染年限土壤中微生物与环境因子进行相关性分析

结果显示:随石油污染年限的延长

土壤微生物群落结构及丰度呈规律性变化.准东油田石油污染土壤当中部分种类微生物和功能基因可作为检测石油污染物老化程度的分子标准.

Abstract

This paper aims to investigate the change rule of bacteria and aromatic dioxygenase gene diversity in oil-contaminated soil with different years from Zhundong oilfield

Xinjiang

China. The oil-contaminated and background soils of three different pollution years were collected in the same oil exploring area

and the soil physical and chemical properties were detected. The V4-V5 hypervariable regions of bacterial 16 S rRNA gene were amplified and sequenced and the aromatic dioxygenase gene clone library were constructed with the different soil samples.The 16 S rRNA gene sequencing results showed that the bacterial community structure and diversity are similarity in different background soils and the bacterial diversity in the soil polluted for 1 year is similar with that in the background soils. There were some differences in bacterial community between the samples of 3 years and 5 years of pollution

but there was little difference within the group respectively. 46 OTUs of aromatic dioxygenase gene were obtained

and the GenBank accession numbers were KY985598-KY985643. After the homologous analysis

the aromatic dioxygenase gene library were divided into five groups

which were phthalate dioxygenase gene

uncultured actinobacterium aromatic oxygenase gene

naphthalene dioxygenase gene

PAH ring-hydroxylating dioxygenase gene and benzoate dioxygenase gene. There were significant differences in the dioxygenase gene types in soils with different oil pollution years. The correlation between microbial community with environmental factors of soils with different pollution years were analyzed. The results showed that the microbial community structure and abundance changed regularly with the increase of pollution years. The paper implied that some microbial species and functional genes can be served as a molecular standard to estimate the degree of aged oil sludge in contaminated soil of Zhundong oilfield.

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