盐穗木miR397a增强转基因拟南芥的低温耐性

魏冉; 张慧珍; 陈方圆; 赵丽娟; 李文辉; 曾幼玲

doi:10.13568/j.cnki.651094.651316.2022.03.05.0001

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盐穗木miR397a增强转基因拟南芥的低温耐性

生命科学与技术 | 更新时间：2026-01-21

- 盐穗木miR397a增强转基因拟南芥的低温耐性
- 盐穗木miR397a增强转基因拟南芥的低温耐性
- 新疆大学学报（自然科学版中英文） 2023年40卷第1期页码：87-94
- 作者机构：
  
  新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室
- 作者简介：
- 基金信息：
  
  国家自然科学基金“荒漠盐生植物盐穗木两个microRNA与靶标基因的耐盐抗旱功能研究”（31760071）
- DOI：10.13568/j.cnki.651094.651316.2022.03.05.0001
  中图分类号： Q943.2
- 纸质出版：2023
- 稿件说明：
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[1]魏冉,张慧珍,陈方圆,等.盐穗木miR397a增强转基因拟南芥的低温耐性[J].新疆大学学报(自然科学版)(中英文),2023,40(01):87-94.
[1]魏冉,张慧珍,陈方圆,等.盐穗木miR397a增强转基因拟南芥的低温耐性[J].新疆大学学报(自然科学版)(中英文),2023,40(01):87-94. DOI： 10.13568/j.cnki.651094.651316.2022.03.05.0001.

DOI：10.13568/j.cnki.651094.651316.2022.03.05.0001.

摘要

盐穗木（Halostachys caspica）是耐盐性极强的苋科藜属盐生植物．mi RNA是长度为21～24 nt的内源性非编码小RNA，通常在转录后水平负调控靶基因的表达介导多种生物学过程．从盐穗木高盐胁迫下根的小RNA文库和转录组数据中，克隆盐穗木mi R397a(HcmiR397a)前体序列和漆酶Hc LACs(Laccase

LAC)为预测的靶基因．通过实验的方法鉴定HcmiR397a与HcLACs的靶向关系及HcmiR397a在多种非生物胁迫下的表达和HcmiR397a的转基因拟南芥在低温胁迫下的功能．研究结果表明：（1）烟草瞬时表达体系和5’RLM-RACE技术确定HcmiR397a与HcLAC17之间存在靶向切割，具体的切割位点位于HcmiR397a的第10～11碱基处；（2）q RT-PCR检测的结果显示盐、干旱和低温胁迫下，盐穗木同化枝HcmiR397a受到显著诱导；（3）异源表达HcmiR397a的转基因拟南芥表现出较强的低温耐性．

Abstract

Halostachys caspica is a halophyte belonging to Chenopodium of Amaranthaceae with extreme salt tolerance. mi RNA is an endogenous small non-coding RNA with a length of 21～24 nt and participates in a variety of biological processes by negative regulating the expression of target genes at the post transcriptional level. The mi R397a precursor were screened

cloned and LAC genes(Laccase

LAC) were predicted from the H.caspica root small RNA libraries under high salinity and this species' s transcriptome data. This paper aims to identify the targeting relationship between HcmiR397a and Hc LACs by experimental methods

the expression of HcmiR397a in the H. caspica branches under various abiotic stresses and the function of HcmiR397a transgenic Arabidopsis under low temperature stress. The results were as follows:(1) Tobacco transient expression system and 5'RLM-RACE technology confirmed that there was a targeted cleavage between HcmiR397a and HcLAC17

and the specific cleavage site was located at the 10th～11th base of HcmiR397a;(2) The results of q RT-PCR showed that HcmiR397a was significantly induced under salt

drought and low temperature stress;(3) Transgenic Arabidopsis heterologously expressing HcmiR397a improved cold tolerance

compared to the wild type.

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