刺山柑果实正丁醇提取物对树突状细胞成熟的影响及机制研究

阿力木·艾麦尔; 慕丽红; 刘晓颖; 韩鹏; 王新绘; 李金耀

doi:10.13568/j.cnki.651094.651316.2022.06.23.0001

您当前的位置：

首页 >

文章列表页 >

刺山柑果实正丁醇提取物对树突状细胞成熟的影响及机制研究

生命科学与技术 | 更新时间：2026-01-23

- 刺山柑果实正丁醇提取物对树突状细胞成熟的影响及机制研究
- 刺山柑果实正丁醇提取物对树突状细胞成熟的影响及机制研究
- 新疆大学学报（自然科学版中英文） 2023年40卷第3期页码：331-341
- 作者机构：
  
  1. 新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室
  2. 新疆大学生态与环境学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金“维药刺山柑耐受佐剂筛选及其在类风湿性关节炎治疗性疫苗研制中的应用”（31760260）;新疆维吾尔自治区重点研发任务专项“新疆特色中草药及联合策略治疗自身免疫病关键技术研发及应用”（2022B03002-2）
- DOI：10.13568/j.cnki.651094.651316.2022.06.23.0001
  中图分类号： R285
- 纸质出版：2023
- 稿件说明：
移动端阅览
[1]阿力木·艾麦尔,慕丽红,刘晓颖,等.刺山柑果实正丁醇提取物对树突状细胞成熟的影响及机制研究[J].新疆大学学报(自然科学版)(中英文),2023,40(03):331-341.
[1]阿力木·艾麦尔,慕丽红,刘晓颖,等.刺山柑果实正丁醇提取物对树突状细胞成熟的影响及机制研究[J].新疆大学学报(自然科学版)(中英文),2023,40(03):331-341. DOI： 10.13568/j.cnki.651094.651316.2022.06.23.0001.

DOI：10.13568/j.cnki.651094.651316.2022.06.23.0001.

摘要

为探讨刺山柑果实正丁醇相(Capparis spinosa L. fruit n-Butanol Extract

CSBE)对小鼠树突状细胞(Dendritic Cell

DC)成熟的影响及其作用机理，用1、2、3 mg·mL-1的CSBE处理DC，采用流式细胞术和ELISA检测DC表面标志物、抗原吞噬能力和细胞因子分泌验证DC成熟度．采用液相色谱-质谱联用(LC-MS)鉴定CSBE抗炎活性成分，基于网络药理学分析其在类风湿性关节炎(Rheumatoid Arthritis

RA)的相关靶点．利用String数据库和Cytoscape 3.7.2软件构建关键靶点蛋白质-蛋白质互作(Protein-Protein Interaction

PPI)网络，利用Metascape平台进行GO和KEGG富集分析并用western blot检测相关蛋白的表达．实验结果显示：CSBE单独处理时对DC表面共刺激分子和细胞因子没有显著影响，但显著抑制了细菌脂多糖(Lipopolysaccharides

LPS)诱导的DC表面分子CD40和CD86的表达、细胞因子TNF-α和IL-6的分泌以及基质金属蛋白酶MMP9和MMP13的表达，增加了抗原吞噬能力．LC-MS正离子模式下有49种抗炎化合物，负离子模式下有42种抗炎化合物．抗炎化合物在RA中共有核心靶点包括AKT1、TNF、SRC及MMP9等，其中有机酸类化合物主要关联辅助性T细胞的分化、Th17辅助性细胞的分化、Th17型免疫反应等Th17相关的生物学过程，涉及PI3K-AKT信号通路、Th17细胞分化、HIF-1信号通路和IL-17信号通路等类风湿性关节炎相关的信号通路．CSBE能够抑制DC的成熟及促炎细胞因子的表达，通过降低MMP表达抑制DC迁移，从而发挥免疫抑制及治疗RA的功能．

Abstract

This study investigated the effect and mechanism of Capparis spinosa L. fruit n-butanol extract(CSBE)on the maturation of dendritic cell(DC). DC was treated with 1

3 mg·mL-1 CSBE. DC surface molecule expression

antigen phagocytosis and cytokine secretion were determined by fow cytometry and ELISA to verify DC maturity. Liquid chromatography-mass spectrometry(LC-MS) was used to identify anti-infammatory components of CSBE and network pharmacology was utilized to analyze their targets related to rheumatoid arthritis(RA).String database and Cytoscape 3.7.2 software were utilized to construct protein-protein interaction(PPI) network maps of the key targets

and Metascape platform was used for GO and KEGG enrichment analysis and western blot was used to identify the expression of related proteins. The experiment results showed that CSBE alone had no significant effect on the expression of DC co-stimulatory molecules and cytokines

but significantly inhibited the expression of CD40 and CD86

matrix metalloproteinases MMP9 and MMP13

and the secretion of TNF-αand IL-6 induced by bacterial lipopolysaccharides(LPS). CSBE also increased antigen phagocytosis of DC. There were 49 anti-infammatory compounds in the positive ion mode of LC-MS

and 42 anti-infammatory compounds in the negative ion mode. Common core targets include AKT1

TNF

SRC and MMP9. Among them

organic acids are mainly associated with Th17-related biological processes such as helper T cell differentiation

Th17 helper cell differentiation

Th17 type immune response

et al.. It involves PI3K-AKT signaling pathway

Th17 cell differentiation

HIF-1 signaling pathway

and IL-17 signaling pathway related to RA. CSBE can inhibit the maturation of DC and the expression of pro-infammatory cytokines

and inhibit DC migration by reducing the expression of MMP

thus exerting the function of immunosuppression and treatment of RA.

关键词

Keywords

references

YAO C L, TSENG T Y. The synergistic and enhancive effects of IL-6 and M-CSF to expand and differentiate functional dendritic cells from human monocytes under serum-free condition[J]. Journal of Biological Engineering, 2023, 17(1):6-21.

MITCHELL D, CHINTALA S, DEY M. Plasmacytoid dendritic cell in immunity and cancer[J]. Journal of Neuroimmunology,2018, 322:63-73.

GALLUCCI S, LOLKEMA M, MATZINGER P. Natural adjuvants:endogenous activators of dendritic cells[J]. Nature Medicine,1999, 5(11):1249-1255.

O’NEILL L A J, GOLENBOCK D, BOWIE A G. The history of toll-like receptors:redefining innate immunity[J]. Nature Reviews Immunology, 2013, 13(6):453-460.

BAGWE P, BAJAJ L, RIKHAV P, et al. Assessment of in vitro immunostimulatory activity of an adjuvanted whole-cell inactivated neisseria gonorrhoeae microparticle vaccine formulation[J]. Vaccines, 2022, 10(7):983-998.

TURNER S, RAISLEY B, ROACH K, et al. Gram-positive bacteria cell wall peptidoglycan polymers activate human dendritic cells to produce IL-23 and IL-1β and promote TH17 cell differentiation[J]. Microorganisms, 2023, 11(1):173-185.

PIACENTE F, BOTTERO M, BENZI A, et al. Neuroprotective potential of dendritic cells and sirtuins in multiple sclerosis[J].International Journal of Molecular Sciences, 2022, 23(8):4352-4373.

KHAN S, GREENBERG J D, BHARDWAJ N. Dendritic cells as targets for therapy in rheumatoid arthritis[J]. Nature Reviews Rheumatology, 2009, 5(10):566-571.

LI J Y, WANG X H, WANG W L, et al. Pleurotus ferulae water extract enhances the maturation and function of murine bone marrow-derived dendritic cells through TLR4 signaling pathway[J]. Vaccine, 2015, 33(16):1923-1933.

AZEGULI H, LI J Y, ZHOU F F, et al. Capparis spinosa fruit ethanol extracts exert different effects on the maturation of dendritic cells[J]. Molecules, 2017, 22(1):97-103.

邓丹丹,阿地拉·艾皮热,阿则古丽·哈木提,等.刺山柑果实总生物碱粗提物对小鼠树突状细胞成熟的影响[J].中华微生物学和免疫学杂志, 2018, 38(12):922-930.

LIU L T, YANG X, YUAN P F, et al. In vitro and in vivo dendritic cell immune stimulation effect of low molecular weight fucoidan from New Zealand Undaria pinnatifida[J]. Marine Drugs, 2022, 20(3):197-212.

AIPIRE A, MAHABATI M, CAI S S, et al. The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis[J].Peer J, 2020, 8:8294-8311.

GFELLER D, GROSDIDIER A, WIRTH M, et al. Swisstargetprediction:a web server for target prediction of bioactive small molecules[J]. Nucleic Acids Research, 2014, 42(W1):W32-W38.

UMBREEN H, ZHANG X, TANG K T, et al. Regulation of myeloid dendritic cells by synthetic and natural compounds for the treatment of rheumatoid arthritis[J]. International Journal of Molecular Sciences, 2022, 24(1):238-250.

杨鑫,李源力,蒋萍,等.杨梅素通过PI3K/AKT/NF-κB信号通路对骨性关节炎发展的影响[J].暨南大学学报(自然科学与医学版), 2020, 41(1):48-57.

赵铤,赵全丰,丁汉琳.舒芬太尼通过mi R-365-3p/AKT1抑制胃癌细胞迁移、侵袭的研究[J].中国药师, 2021, 24(8):450-456.

邓艳玲,翁文玉,招泽豪,等.黄芪三仙汤抗绝经后骨质疏松作用机制的研究[J].广州中医药大学学报, 2022, 39(4):898-907.

WEI X H, ZHANG B P, LIANG X, et al. Higenamine alleviates allergic rhinitis by activating AKT1 and suppressing the EGFR/JAK2/c-JUN signaling[J]. Phytomedicine, 2021, 86:153565.

HEDIN U, MATIC L P. Recent advances in therapeutic targeting of inflammation in atherosclerosis[J]. Journal of Vascular Surgery,2018, 69(3):944-951.

曹婷,张朝,胡玲,等. TNFα诱导STAT3信号转导的分子机制研究[J].中国细胞生物学学报, 2022, 44(2):239-247.

LORENZO-GOMEZ I, NOGUEIRA-RECALDE U, OREIRO N, et al. HSP90AA1 is a biomarker associated with defective autophagy in osteoarhritis[J]. Osteoarthritis and Cartilage, 2020, 28(S1):319.

邹月,赵海南,刘丹丹,等.儿咳灵糖浆对支气管哮喘模型小鼠Caspase-3, TNF-α表达的影响[J].新疆医科大学学报, 2021, 44(10):1128-1132.

张荣凯,叶志强,陈琰,等.早期骨关节炎软骨下骨趋化因子信号通路的表达[J].中国组织工程研究, 2013, 17(11):1925-1930.

王苹苹,孔繁平,陈学群,等.低氧细胞应激的HIF-1信号通路[J].浙江大学学报(医学版), 2011, 40(5):559-566.

陈丽,李芳,文李娜,等. Th17/Treg失衡与肥胖人群中类风湿关节炎患病率升高的相关性[J].中国临床研究, 2022, 35(3):415-417.

杨雪娜,熊咏民.基质金属蛋白酶在骨关节疾病中的研究进展[J].国外医学(医学地理分册), 2019, 40(4):470-474.

侯文哲,刘时华,钟平.缺血性脑白质病变与基质金属蛋白酶-3、基质金属蛋白酶-9的临床关系研究[J].安徽医药, 2022, 26(3):505-509.

NIU G, WRIGHT K L, HUANG M, et al. Constitutive STAT3 activity up-regulates VEGF expression and tumor angiogenesis[J].Oncogene, 2002, 21(13):2000-2008.

浏览量

334

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

基于网络药理学探讨圣草酚对树突状细胞成熟的影响及分子机制

新疆祖母绿与哥伦比亚祖母绿颜色特征的对比研究