HepG2细胞胰岛素抵抗模型建立及在筛选天然有效多糖中的应用

姜慰; 史茜; 刘晓颖; 付玉兰; 李金耀; 王为兰

doi:10.13568/j.cnki.651094.651316.2022.12.22.0001

您当前的位置：

首页 >

文章列表页 >

HepG2细胞胰岛素抵抗模型建立及在筛选天然有效多糖中的应用

生命科学与技术 | 更新时间：2026-01-21

- HepG2细胞胰岛素抵抗模型建立及在筛选天然有效多糖中的应用
- HepG2细胞胰岛素抵抗模型建立及在筛选天然有效多糖中的应用
- 新疆大学学报（自然科学版中英文） 2023年40卷第4期页码：461-466
- 作者机构：
  
  1. 新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室
  2. 新疆生产建设兵团医院检验科
- 作者简介：
- 基金信息：
  
  新疆维吾尔自治区自然科学青年基金“肉苁蓉苯乙醇苷改善脂肪细胞胰岛素抵抗的作用及机理研究”（2020D01C070）;新疆维吾尔自治区重点实验室开放课题“管花肉苁蓉苯乙醇苷调控代谢性炎症改善脂肪胰岛素抵抗的作用及其机制”（2021D04019）
- DOI：10.13568/j.cnki.651094.651316.2022.12.22.0001
  中图分类号： R965.1
- 纸质出版：2023
- 稿件说明：
移动端阅览
[1]姜慰,史茜,刘晓颖,等.HepG2细胞胰岛素抵抗模型建立及在筛选天然有效多糖中的应用[J].新疆大学学报(自然科学版)(中英文),2023,40(04):461-466.
[1]姜慰,史茜,刘晓颖,等.HepG2细胞胰岛素抵抗模型建立及在筛选天然有效多糖中的应用[J].新疆大学学报(自然科学版)(中英文),2023,40(04):461-466. DOI： 10.13568/j.cnki.651094.651316.2022.12.22.0001.

DOI：10.13568/j.cnki.651094.651316.2022.12.22.0001.

摘要

探讨高糖、地塞米松、胰岛素诱导肝细胞HepG2胰岛素抵抗模型（IR-HepG2）的建立方法，检测杏鲍菇多糖、红枣多糖和枸杞多糖体外改善肝细胞胰岛素抵抗的作用，评估3种多糖的降糖效果．选择不同浓度的地塞米松、胰岛素及两者联合作用，在高糖培养条件下诱导HepG2胰岛素抵抗，MTT法检测不同诱导剂对HepG2细胞活性的影响，葡萄糖氧化酶法测定细胞葡萄糖消耗量，同时检测去除诱导剂后，IR-HepG2细胞模型的稳定性．以3种天然多糖处理IR-HepG2细胞，检测其对细胞活力及胰岛素抵抗的影响．结果表明：在高糖培养条件下，诱导剂为地塞米松（3.75μmol/L）联合胰岛素（0.001μmol/L）可成功诱导IR-HepG2细胞模型，且对细胞活性无显著影响，模型能够在72 h内保持稳定．枸杞多糖具有更好的降糖效果．

Abstract

To explore the establishment of insulin resistance model of HepG2(IR-HepG2) induced by high glucose

dexamethasone and insulin

to detect the effect of Pleurotus eryngii polysaccharides

Ziziphus jujuba polysaccharides and Lycium barbarum polysaccharides on improving insulin resistance of liver cells in vitro

and to analyze the effect of three polysaccharides on hypoglycemic. Different concentrations of dexamethasone

insulin and their combination were selected to induce the insulin resistance of HepG2 cells in high glucose culture. MTT method was used to detect the effect of different inducers on the activity of HepG2 cells. Glucose oxidase method was used to detect the glucose content in the supernatant of cell culture. At the same time

the stability of IR-HepG2 cell model was detected after removing inducers. IR-HepG2 cells were treated with three kinds of natural polysaccharides at different concentrations. The effects of polysaccharides on cell viability and insulin resistance were detected by MTT and glucose oxidase methods respectively. Dexamethasone(3.75 μmol/L) combined with insulin(0.001μmol/L) can successfully induce IR-HepG2 cell model in high glucose culture condition

and has no significant effect on cell activity

and the model can keep stable within 72 hours. Lycium barbarum polysaccharides had better hypoglycemic effects in vitro.

关键词

Keywords

references

WANG Y T, LIN D B, WANG X L, et al. The impact of a novel peach gum-derived polysaccharide on postprandial blood glucose control in streptozotocin-induced diabetic mice[J]. International Journal of Biological Macromolecules, 2017, 98:379-386.

KOKIL G R, VEEDU R N, RAMM G A, et al. Type 2 diabetes mellitus:limitations of conventional therapies and intervention with nucleic acid-based therapeutics[J]. Chemical Reviews, 2015, 115(11):4719-4743.

CHEN L, LIN X J, TENG H. Emulsions loaded with dihydromyricetin enhance its transport through Caco-2 monolayer and improve anti-diabetic effect in insulin resistant HepG2 cell[J]. Journal of Functional Foods, 2020, 64:103672.

ARNOLDS S, HEISE T, FLACKE F, et al. Common standards of basal insulin titration in type 2 diabetes[J]. Journal of Diabetes Science&Technology, 2013, 7(3):771-788.

KARIYA T, TAKAHASHI K, ITAGAKI D, et al. Scallop mantle extract inhibits insulin signaling in HepG2 cells[J]. Food Science&Nutrition, 2019, 7(6):2159-2166.

HU X, WANG S, XU J, et al. Triterpenoid saponins from Stauntonia chinensis ameliorate insulin resistance via the AMP-activated protein kinase and IR/IRS-1/PI3K/Akt pathways in insulin-resistant HepG2 cells[J]. International Journal of Molecular Sciences,2014, 15(6):10446-10458.

THOMSEN S K, ANNE R, BENOIT H, et al. Type 2 diabetes risk alleles in PAM impact insulin release from human pancreaticβ-cells[J]. Nature Genetics, 2018, 50(8):1122-1131.

SUKALINGAM K, GANESAN K, PONNUSAMY K. Evaluation of antidiabetic activity of polyherbal formulations on type 2diabetic patients:a single blinded randomized study[J]. International Journal of Medical Sciences, 2015, 2:90-98.

赵婧,袁驰,周春丽,等.南瓜多糖降血糖作用研究进展[J].食品研究与开发, 2014, 35(7):108-110.

DO AMARAL A E, PETKOWICZ C L O, MERCE A L R, et al. Leishmanicidal activity of polysaccharides and their oxovanadium(IV/V)complexes[J]. European Journal of Medicinal Chemistry:Chimie Therapeutique, 2015, 90:732-741.

ZONG A Z, CAO H Z, WANG F S. Anticancer polysaccharides from natural resources:a review of recent research[J]. Carbohydrate Polymers, 2012, 90(4):1395-1410.

DONG B X, HADINOTO K. Direct comparison between millifluidic and bulk-mixing platform in the synthesis of amorphous drug-polysaccharide nanoparticle complex[J]. International Journal of Pharmaceutics, 2017, 523(1):42-51.

LEAL-LOPES C, VELLOSO F J, CAMPOPIANO J C, et al. Roles of commensal microbiota in pancreas homeostasis and pancreatic pathologies[J]. Journal of Diabetes Research, 2015, 2015:284680.

KASSEM M, PHILIPPE F, ISABELLE W. Adipose tissue in obesity-related inflammation and insulin resistance:cells, cytokines,and chemokines[J]. International Scholarly Research Notices, 2013, 2013:139239.

CHENG F E, LIN H, XIAO Y, et al. D-chiro-inositol ameliorates high fat diet-induced hepatic steatosis and insulin resistance via PKCε-PI3K/AKT pathway[J]. Journal of Agricultural&Food Chemistry, 2019, 67(21):5957-5967.

DING X Q, JIAN T Y, WU Y X, et al. Ellagic acid ameliorates oxidative stress and insulin resistance in high glucose-treated HepG2 cells via mi R-223/keap1-Nrf2 pathway[J]. Biomedicine&Pharmacotherapy, 2019, 110:85-94.

ZHAO W J, LI A Y, FENG X, et al. Metformin and resveratrol ameliorate muscle insulin resistance through preventing lipolysis and inflammation in hypoxic adipose tissue[J]. Cellular Signalling, 2016, 28(9):1401-1411.

FORETZ M, GUIGAS B, BERTRAND L, et al. Metformin:from mechanisms of action to therapies[J]. Cell Metabolism, 2014,20(6):953-966.

JIN X, WANG Q L, YANG X, et al. Chemical characterisation and hypolipidaemic effects of two purified Pleurotus eryngii polysaccharides[J]. International Journal of Food Science&Technology, 2018, 53(10):2298-2307.

JING L J, CUI G W, FENG Q, et al. Evaluation of hypoglycemic activity of the polysaccharides extracted from Lycium barbarum[J]. African Journal of Traditional, Complementary and Alternative Medicines, 2009, 6(4):579-584.

ZHAO Y, YANG X B, REN D Y, et al. Preventive effects of jujube polysaccharides on fructose-induced insulin resistance and dyslipidemia in mice[J]. Food&Function, 2014, 5(8):1771-1778.

浏览量

1588

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

枸杞干红酒对小鼠抗疲劳、抗氧化作用的影响

BMPs在脂肪胰岛素抵抗中的研究进展