重组溶瘤单纯疱疹病毒对小鼠结肠癌移植瘤的抑制作用

朱彦斌; 彭瑞娟; 张磊; 张新强; 马正海

doi:10.13568/j.cnki.651094.651316.2023.05.24.0001

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重组溶瘤单纯疱疹病毒对小鼠结肠癌移植瘤的抑制作用

生命科学与技术 | 更新时间：2026-01-21

- 重组溶瘤单纯疱疹病毒对小鼠结肠癌移植瘤的抑制作用
- 重组溶瘤单纯疱疹病毒对小鼠结肠癌移植瘤的抑制作用
- 新疆大学学报（自然科学版中英文） 2024年41卷第1期页码：87-92
- 作者机构：
  
  新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室
- 作者简介：
- 基金信息：
  
  国家自然科学基金“表达GM-CSF和IL-12的1型单纯疱疹病毒载体系统的研究”（31140018）
- DOI：10.13568/j.cnki.651094.651316.2023.05.24.0001
  中图分类号： R735.35
- 纸质出版：2024
- 稿件说明：
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[1]朱彦斌,彭瑞娟,张磊,等.重组溶瘤单纯疱疹病毒对小鼠结肠癌移植瘤的抑制作用[J].新疆大学学报(自然科学版)(中英文),2024,41(01):87-92.
[1]朱彦斌,彭瑞娟,张磊,等.重组溶瘤单纯疱疹病毒对小鼠结肠癌移植瘤的抑制作用[J].新疆大学学报(自然科学版)(中英文),2024,41(01):87-92. DOI： 10.13568/j.cnki.651094.651316.2023.05.24.0001.

DOI：10.13568/j.cnki.651094.651316.2023.05.24.0001.

摘要

探讨表达p53和IL-12基因的重组溶瘤单纯疱疹病毒(oncolytic Herpes Simplex Virus

oHSV)MH1004和MH-1006对小鼠结肠癌移植瘤的抑制作用．分别以Western blot和ELISA检测MH1004和MH1006感染细胞中治疗基因p53和IL-12的表达．以结肠癌细胞CT26构建小鼠皮下移植瘤模型，瘤内注射HSV-wt、HSV空载体MH1001和MH1005以及oHSV MH1004和MH1006，观察oHSV对小鼠肿瘤生长、存活率及肿瘤大小的影响，结果显示：Western blot可检测到MH1004感染细胞中p53蛋白的表达；ELISA检测表明MH1006在感染细胞早期表达IL-12蛋白．荷瘤小鼠治疗第21d

MH1004、MH1005和MH1006治疗组肿瘤体积依次为2 820.69±2 539.35 mm3、2 127.31±2 017.02 mm3和1 414.36±1 639.19 mm3，均显著小于Mock组(7 682.92±2 648.74 mm3)(P <0.01)．治疗42 d时，MH1004、MH1005和MH1006组均有1只小鼠肿瘤完全消失．小鼠生存率结果表明：MH1004(100%)和MH1006组(100%)显著高于MH1001(66.67%)、MH1005(66.67%)、HSV-wt(50%)和Mock组(50%)(P <0.05)．以上研究说明MH1005是一种理想的复制型溶瘤病毒载体；表达p53和IL-12的oHSV能够有效抑制小鼠体内结肠癌肿瘤的生长，是一种潜在的结肠癌治疗新方法．

Abstract

To study the inhibitory effect of the recombinant oncolytic herpes simplex virus(oHSV) MH1004and MH1006 expressing p53 and IL-12 genes on the transplanted colon cancer tumors in mice. Western blot and ELISA were used to detect the expression of therapeutic gene p53 and IL-12 in the cells infected with MH1004and MH1006 respectively; Colon cancer cell CT26 was used to construct the subcutaneous transplanted tumor model in mice. HSV-wt

HSV empty viral vector MH1001 and MH1005

as well as oHSV MH1004 and MH1006were injected into the tumor to observe the effect of the oHSVs on tumor growth

survival rate and tumor size in the mice. The results were shown as below

p53 protein was detected by Western blot in the cells infected with MH1004

and IL-12 protein was detected by ELISA in the cells infected with MH1006 in the early stage. At day21 of treatment for tumor bearing mice

the tumor volumes of MH1004

MH1005 and MH1006 treatment groups were 2 820.69±2 539.35 mm3

2 127.31±2 017.02 mm3 and 1 414.36±1 639.19 mm3 respectively

which were all significantly smaller than that of Mock group(7 682.92±2 648.74 mm3)(P <0.01). At day 42 of the treatment

the tumor in one of the mice from MH1004

MH1005 and MH1006 groups all disappeared respectively

and the survival rates of mice of MH1004(100%) and MH1006(100%) groups were significantly higher than that of MH1001(66.67%)

MH1005(66.67%)

HSV-wt(50%) and Mock(50%) groups(P <0.05). This study suggests MH1005 is an ideal replication oncolytic viral vector; oHSV expressing p53 and IL-12 can effectively inhibit the growth of colon cancer tumors in mice

which is a potential new method for colon cancer treatment.

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