基于纳米金/多孔硅基底的荧光标记法对生物分子的检测

何蕾

doi:10.13568/j.cnki.651094.651316.2020.10.16.0002

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基于纳米金/多孔硅基底的荧光标记法对生物分子的检测

数理科学 | 更新时间：2026-01-21

- 基于纳米金/多孔硅基底的荧光标记法对生物分子的检测
- 基于纳米金/多孔硅基底的荧光标记法对生物分子的检测
- 新疆大学学报（自然科学版中英文） 2021年38卷第2期页码：159-162
- 作者机构：
  
  新疆大学物理科学与技术学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金(61575168)
- DOI：10.13568/j.cnki.651094.651316.2020.10.16.0002
  中图分类号： O657.3;TP212
- 纸质出版：2021
- 稿件说明：
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[1]何蕾.基于纳米金/多孔硅基底的荧光标记法对生物分子的检测[J].新疆大学学报(自然科学版)(中英文),2021,38(02):159-162+167.
[1]何蕾.基于纳米金/多孔硅基底的荧光标记法对生物分子的检测[J].新疆大学学报(自然科学版)(中英文),2021,38(02):159-162+167. DOI： 10.13568/j.cnki.651094.651316.2020.10.16.0002.

DOI：10.13568/j.cnki.651094.651316.2020.10.16.0002.

摘要

为制做一种低成本、易标记、稳定性好、灵敏度高的生物传感器

本文制备了一种具有较强荧光信号及生物兼容性的多孔硅生物传感器材料.利用纳米金的等离子体效应增强多孔硅中荧光标记物的荧光信号

在纳米金修饰的多孔硅中采用罗丹明6G荧光标记法对生物分子进行检测.通过链接生物荧光探针后荧光信号强度的变化实现生物浓度检测

其检测极限为121 nM

对生物大分子成功实现了低成本、易标记、稳定性好、灵敏度高的检测.

Abstract

The purpose of this study is to prepare a low-cost

easy labeling

good stability and high sensitivity biosensor

a porous silicon biosensor material with strong fluorescence signal and biocompatibility was successfully prepared

the plasma effect of gold nanoparticles is used to enhance the fluorescence signal of fluorescent markers in porous silicon

Rhodamine 6 G fluorescence labeling method was used to detect biomolecules in modified porous silicon by gold nanoparticles.Through the change of fluorescence signal intensity after linking the biological fluorescence probe

the biological concentration can be detected

the detection limit is 121 nM

it can detect biomacromolecules with low cost

easy labeling

good stability and high sensitivity.

关键词

Keywords

references

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