This research aim was to investigate whether cytokines:Interleukin 1β(IL β)

Interleukin 2(IL 2) and Interleukin 6(IL 6) affect the subsequent development of ovine IVF derved embryos in a droplet of synthetic oviduct fluid (SOF) medium with or without addition of different doses of cytokines

These results shows:1) There were significant increases in the developmental rate of early IVF derived embryos to hatching blastocysts with addition of IL 1β in doses of 500IU/ml

1000IU/ml(P<0 05). With addition of IL 1β in dose of 50IU/ml

there is no difference of the hatching rate of ovine embryo comparing with the control group.2) It showed the significant increases in the developmental rate of early IVF derived embryos to hatching blastocysts with addition of IL 6 in dose of 50IU/ml(p<0 05). With 500 and 1000IU/ml addition of IL 6

it is no differences comparing with the control group. 3) There were no differences in the developmental rate of early IVF derived embryos to hatching blastocysts with the addition of IL 2 in doses of 50IU/ml

500IU/ml

100IU/ml.These results suggest that interleukins added in cultured medium may contrebute to changes the culture condition of ovine embryos and affect the development of sheep embryo in vitro.