新疆妇女宫颈癌组织中HPV-16E1/dup突变体分布的研究

侯向前; 史茜; 朱开春; 玛依努尔·尼亚孜; 马正海

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新疆妇女宫颈癌组织中HPV-16E1/dup突变体分布的研究

更新时间：2026-01-21

- 新疆妇女宫颈癌组织中HPV-16E1/dup突变体分布的研究
- Journal of Xinjiang University (Natural Science Edition in Chinese and English) Vol. 29, Issue 4, Pages: 384-389(2012)
- 作者机构：
  
  1. 新疆大学生命科学与技术学院
  2. 新疆自治区人民医院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.33
- Published：2012
- 稿件说明：
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[1]侯向前,史茜,朱开春,等.新疆妇女宫颈癌组织中HPV-16 E1/dup突变体分布的研究[J].新疆大学学报(自然科学版),2012,29(04):384-389+505.

侯向前, 史茜, 朱开春, et al. 新疆妇女宫颈癌组织中HPV-16E1/dup突变体分布的研究[J]. Journal of Xinjiang University (Natural Science Edition in Chinese and English), 2012, 29(4): 384-389.
[1]侯向前,史茜,朱开春,等.新疆妇女宫颈癌组织中HPV-16 E1/dup突变体分布的研究[J].新疆大学学报(自然科学版),2012,29(04):384-389+505. DOI：

侯向前, 史茜, 朱开春, et al. 新疆妇女宫颈癌组织中HPV-16E1/dup突变体分布的研究[J]. Journal of Xinjiang University (Natural Science Edition in Chinese and English), 2012, 29(4): 384-389. DOI：

摘要

目的检测HPV-16 1311～1773位63bp重复序列插入突变的E1基因(E1/dup)在宫颈炎、宫颈上皮内瘤变(cervical intraepithelial neoplasia

CIN)及宫颈癌病变组织中的分布及病毒的整合状态

探讨该突变对病毒致癌活性的影响.方法收集维吾尔族和汉族宫颈炎、CIN和宫颈癌临床组织标本215例

提取组织DNA

PCR检测E1/dup突变体以及病毒E1/E2基因的完整性.结果新疆地区215例宫颈病变组织中HPV-16 E1检出率为60.00%(129/215)

宫颈炎、CIN及宫颈癌病样中HPV-16 E1的检出率分别为31.15%(19/61)、49.21%(31/63)和86.81%(79/91).在HPV-16E1阳性病样中

HPV-16 E1/dup突变率为37.21%(48/129)

宫颈炎、CIN和宫颈癌病样中E1/dup突变率分别为15.79%、22.58%和48.10%

E1/dup突变率在宫颈癌中显著高于宫颈炎(P<0.05)和CIN(P<0.05).E1/dup突变率在汉族和维吾尔族宫颈癌病样中分别为40.00%(6/15)和50.00%(32/64)

两者无显著性差异(P>0.05).在HPV-16 E1阳性病样中病毒整合率为51.72%(60/116)

E1/dup突变病样中HPV16整合率[69.05%(29/42)]明显高于未发生E1/dup突变的病样[41.89%(31/74)](P<0.05).结论新疆地区E1/dup突变率在宫颈癌中显著高于宫颈炎及CIN

E1/dup与病毒致癌活性及病毒整合相关.

Abstract

Objective To detect the distribution of HPV-16 E1 gene mutant with the insertion of 63bp nucleotide duplication sequences at position 1311-1373(E1/dup) from clinical tissue specimens of cervicitis

cervical intraepithelial neoplasia(CIN) and cervical cancer

and define the integrated status of HPV-16

then research into the carciongenic potency of E1/dup.Methods 215 clinical tissue specimens of cervicitis

CIN and cervical cancer from Uygur and Han patients were collected

the tissue DNAs were extracted.Then E1/dup mutant and integral E1/E2 were detected by PCR.Results The positive rate of HPV-16 E1 in the 215 specimens was 60.00%(129/215)

and the rates in cervicitis

CIN and cervical cancer were 31.15%(19/61)

49.21%(31/63) and 86.81%(79/91) respectively.In the HPV-16 E1 positive specimens

the rates of E1/dup mutant were 37.21%(48/129)

and the rate of E1/dup mutant from the cervical cancer(48.10%) was significantly higher than that from the cervicitis(15.79%)(P<0.05) and CIN(22.58%)(P<0.05).The rates of E1/dup mutant from Han cervical cancer and Uygur cervical cancer specimens were 40.00%(6/15) and 50.00%(32/64) respectively

there were no significant differences between of them(P>0.05).The rate of integrated HPV-16 from the E1 positive specimens was 51.72%(60/119)

and the rate[69.05%(29/42)] from the E1/dup Specimens was significantly higher than the rate [41.89%(31/74)] from no-E1/dup Specimens.Conclusion The rate of E1/dup mutant from the cervical cancer was significantly higher than that from the cervicitis and CIN

and E1/dup mutant was associated with the carciongenic potency and integrated status of HPV-16.

关键词

Keywords

references

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