甜瓜抗病基因同源序列的克隆与分析

王贤磊; 高兴旺; 张铁钢; 王文林; 熊丽曼; 韩瑞; 李冠

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甜瓜抗病基因同源序列的克隆与分析

更新时间：2026-01-21

- 甜瓜抗病基因同源序列的克隆与分析
- Journal of Xinjiang University (Natural Science Edition in Chinese and English) Vol. 28, Issue 2, Pages: 136-144(2011)
- 作者机构：
  
  新疆大学生命科学与技术学院
- 作者简介：
- 基金信息：
- DOI：
  CLC： S652
- Published：2011
- 稿件说明：
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[1]王贤磊,高兴旺,张铁钢,等.甜瓜抗病基因同源序列的克隆与分析[J].新疆大学学报(自然科学版),2011,28(02):136-144.

王贤磊, 高兴旺, 张铁钢, et al. 甜瓜抗病基因同源序列的克隆与分析[J]. Journal of Xinjiang University (Natural Science Edition in Chinese and English), 2011, 28(2): 136-144.
[1]王贤磊,高兴旺,张铁钢,等.甜瓜抗病基因同源序列的克隆与分析[J].新疆大学学报(自然科学版),2011,28(02):136-144. DOI：

王贤磊, 高兴旺, 张铁钢, et al. 甜瓜抗病基因同源序列的克隆与分析[J]. Journal of Xinjiang University (Natural Science Edition in Chinese and English), 2011, 28(2): 136-144. DOI：

摘要

为了获得新的甜瓜抗病基因同源基因(RGA)

我们根据NBS-LRR类抗病基因保守区设计兼并引物组合

以抗病甜瓜品种基因组DNA为模板进行PCR扩增

经筛选、分类与基因测序

获得7个甜瓜RGA序列

其中821与324两个RGA为本研究首次发现.将得到的序列与已知甜瓜RGA序列进行分析、比对

将重复的RGA合并

通过对不同RGA序列同源分析

发现甜瓜CC-NBS-LRR类与TIR-NBS-LRR类RGA的不同分布特征与不同RGA之间的相互关系

如MRGA10与MRGH5可能起源于同一基因.通过对RGA的遗传分析与比较基因组学分析

发现甜瓜RGA序列与抗病基因之间的对应关系

如抗枯萎病基因Fom-1和抗番木瓜环斑病基因Prv存在于nbs47-3(MRGH11)与MRGH21之间

其中候选抗病基因MRGH8推测为Fom-1;候选抗病基因MRGH9、MRGH10之一为Prv.

Abstract

To isolate resistance gene analogs(RGA) of melon

degenerate primers designed according to NBS-LRR conservative domain of resistance genes were used to amplify with melon genomic DNA.Seven RGAs were cloned and sequenced

out of which 821 and 324 were newly found in this research.All the RGAs were compared to discard the redundant sequence and incorporate the useful information.Different distribution status between CC-NBS-LRRs and TIR-NBS-LRRs were found by the homologous RGA anal-ysis

and also relation of different RGA was found

such as MRGA10 and MRGH5 might be originated from common ancestor.Bioinformation and comparativegenomics method were used to discover the relation between RGAs and disease resistance genes.The Fom-1 gene conferring resistance to Fusarium oxysporum f.sp.melonis race 2 and the Prv conferring resistance to papaya ring spot virus were located between RGAs nbs47-3(MRGH11) and MRGH21

so it was deduced that candidate gene MRGH8 and the Fom-1 were the same gene

while Prv might be the same with one of MRGH9 and MRGH10.

关键词

Keywords

references

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