A new analytical method of flow injection chemiluminescence has been developed for determine tion of trace iron. It is based on the catalysis of Fe(Ⅲ) to the chemiluminescence system of luminolH_2O2- Oxine-EDTA in alkaline medium. The linear range of determination is 1×10-9~1×10-7g/ml. The limit of detection is 1. 2×10-10g/l The relative standard dleviation of the detection is 1.1%. The method has been applied to the determination of iron in some water samples.