新疆双峰驼乳清蛋白组分对人宫颈癌HeLa细胞增殖的抑制作用

杨洁; 王红娟; 豆智华; 李冠

doi:10.13568/j.cnki.651094.2015.01.003

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新疆双峰驼乳清蛋白组分对人宫颈癌HeLa细胞增殖的抑制作用

更新时间：2026-01-21

- 新疆双峰驼乳清蛋白组分对人宫颈癌HeLa细胞增殖的抑制作用
- Journal of Xinjiang University (Natural Science Edition in Chinese and English) Vol. 32, Issue 1, Pages: 11-16(2015)
- 作者机构：
  
  新疆大学生命科学与技术学院
- 作者简介：
- 基金信息：
- DOI：10.13568/j.cnki.651094.2015.01.003
  CLC： TS252.1;R151
- Published：2015
- 稿件说明：
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[1]杨洁,王红娟,豆智华,等.新疆双峰驼乳清蛋白组分对人宫颈癌HeLa细胞增殖的抑制作用[J].新疆大学学报(自然科学版),2015,32(01):11-16.
[1]杨洁,王红娟,豆智华,等.新疆双峰驼乳清蛋白组分对人宫颈癌HeLa细胞增殖的抑制作用[J].新疆大学学报(自然科学版),2015,32(01):11-16. DOI： 10.13568/j.cnki.651094.2015.01.003.

DOI：10.13568/j.cnki.651094.2015.01.003.

摘要

目的:探讨新疆双峰驼乳清蛋白组分对人宫颈癌HeLa细胞增殖的抑制作用.方法:采用四甲基偶氮唑盐(MTT)法测定新疆双峰驼乳蛋白组分对HeLa细胞增殖的抑制作用;荧光染色检测HeLa细胞凋亡的形态变化;倒置显微镜、扫描电镜和透射电镜观察HeLa细胞的形态变化;用流式细胞术检测HeLa细胞凋亡率、线粒体膜电位的变化.结果表明:新疆双峰驼乳清蛋白组分TR35可抑制人宫颈癌HeLa细胞的增殖并诱导其凋亡

并呈时间和剂量依赖性(P<0.01);倒置显微镜、扫描电镜和透射电镜观察到典型的凋亡特征

荧光显微观察到凋亡小体;Annexin-Ⅴ/PI双染检测出现早期凋亡细胞;线粒体膜电位去极化

并将细胞周期阻滞在S期.结论:新疆双峰驼乳清蛋白组分TR35可抑制HeLa细胞的增殖和诱导细胞凋亡

TR35组分诱导细胞凋亡的途径可能与线粒体通路有关.

Abstract

To explore the Xinjiang bactrian camel milk suppressor component that inhibit tumor cell proliferation in vitro and its mechanism

we adopt MTT to screen the whey protein components with anticancer activity. Hela cell morphological changes were studied by inverted microscope

scanning electron microscopy(sem) and transmission electron microscopy and fluorescence microscopy. Flow cytometry was used to detect apoptosis rate

the change of mitochondrial membrane potential in the Hela cells after 24 h treatment with camel whey tumor-suppressor component. Results show that the TR35 fractionation from camel whey have a certain inhibitory effect on cancer cell

and had a certain amount of time-dose dependent manner. TR35 components can induce the apoptosis of Hela cells

the apoptotic body

mitochondrial membrane potential depolarization

and cell cycle arrest in S phase. Under the light microscope and electron microscope showed typical apoptosis characteristics. Annexin-Ⅴ/PI double dye detection appeared early apoptosis cells. Conclusion: Xinjiang bactrian camel whey protein components TR35 inhibits proliferation of esophageal Hela cell

and the induced way may be associated with mitochondrial pathway.

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references

Farah Z.Composition and Characteristics of Camel Milk[J].Journal of Dairy Research,1993,60:603-626

Madany R M.Inhibition effect of camel milk immune proteins against some mastitis-causing bacteria[J].Bio Technology:An Indian Journal,2009,3,30-34.

Liu C,Gelius E,Liu G,et al.Mammalian peptidoglycan recognition protein binds peptidoglycan with high affinity,is expressed in neutrophils,and inhibits bacterial growth[J].Journal of Biological Chemistry,2000,275,24490-24499.

El-Agamy.EI:Effect of heat treatment on camel milk proteins with respect to antimicrobial factors:a comparison with cow’s and buffalo milk[J].Food Chemistry,2000,68:227-232.

Sawaya W N,Kalil J K,et al.Chemical composition and nutritional quality of camel milk[J].Food Sci,1984,49:744-747.

Zhang H,Yao J,Zhao D,et al.Changes in chemical composition of Alxa bactrian camel milk during lactation[J].J Dairy Sci,2005,88(10):3402-3410.

El-Agamy E I,et al.The challenge of cow milk protein allergy[J].Small Rum Res,2007,68:64-72.

Cardoso R R,Santos R M,Cardoso C R,et al.Consumption of camel’s milk by patients intolerant to lactose.A preliminary study[J].Rev Alerg Mex,2010,57(1):26-32.

Darwish,Hebatallah A,Abd Raboh,et al.Camel’s milk alleviates alcohol-induced liver injury in rats[J].Food and Chemical Toxicology,2012,50(5):1377-1383.

潘蕾,王晶,王娟,等.驼乳对慢性肾功能衰竭大鼠的影响[J].食品科学,2010,31(17):376-380.

Ebaid,Hossam,Ahmed,et al.Limiting prolonged inflammation during proliferation and remodeling phases of wound healing in streptozotocin-induced diabetic rats supplemented with camel undenaturedwhey protein[J].BMC Immunology,2013,14:10.1186/1471-2172-14-31.

Habib,Hosam M,Ibrahim,et al.Camel milk lactoferrin reduces the proliferation of colorectal cancer cells and exerts antioxidant and DNA damage inhibitory activities[J].Food Chemistry,2013:141(1):148-152.

王初一,郭建功,哈斯苏荣,等.苏尼特双峰驼鲜驼乳对荷瘤小鼠免疫功能的影响及其抗肿瘤作用[J].食品科学,2009,30(3):270-273.

郭建功,王初一,哈斯苏荣,等.双峰驼自然发酵乳对小鼠腹水型肝癌细胞H22的影响[J].中国奶牛,2009(1):9-11.

Kroemer G,Reed J C.Mitochondrial control of cell death[J].Nature Med,2000,6:513-519.

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