PPARG基因taqSNPs遗传模型及单体型与2型糖尿病的相关性

多力坤·买买提玉素甫; 祖克拉·肉孜; 宋曼殳; 赵飞飞; 伊力哈木·乃扎木

doi:10.13568/j.cnki.651094.2015.04.004

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PPARG基因taqSNPs遗传模型及单体型与2型糖尿病的相关性

更新时间：2026-01-21

- PPARG基因taqSNPs遗传模型及单体型与2型糖尿病的相关性
- Journal of Xinjiang University (Natural Science Edition in Chinese and English) Vol. 32, Issue 4, Pages: 399-409(2015)
- 作者机构：
  
  1. 新疆大学生命科学与技术学院
  2. 首都医科大学公共卫生与家庭医学学院
- 作者简介：
- 基金信息：
- DOI：10.13568/j.cnki.651094.2015.04.004
  CLC： R587.1
- Published：2015
- 稿件说明：
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[1]多力坤·买买提玉素甫,祖克拉·肉孜,宋曼殳,等.PPARG基因taqSNPs遗传模型及单体型与2型糖尿病的相关性[J].新疆大学学报(自然科学版),2015,32(04):399-409+505.
[1]多力坤·买买提玉素甫,祖克拉·肉孜,宋曼殳,等.PPARG基因taqSNPs遗传模型及单体型与2型糖尿病的相关性[J].新疆大学学报(自然科学版),2015,32(04):399-409+505. DOI： 10.13568/j.cnki.651094.2015.04.004.

DOI：10.13568/j.cnki.651094.2015.04.004.

摘要

目的:探讨柯尔克孜人群PPARG基因taq SNPs位点遗传模型及单体型与2型糖尿病的关系.方法:选取柯尔克孜族50例对照和50例2型糖尿病患者分别分为对照和病例组.选择PPARG基因23个SNPs(rs1151999

rs1175540

rs17036242

rs1875796

rs1899951

rs2292101

rs2881654

rs2921190

rs2938397

rs2959272

rs2959273

rs2972162

rs3856806

rs4135247

rs4135275

rs4684846

rs6782475

rs709151

rs7615916

rs7626560

rs9310401

rs9817428

rs1801282)

用MALDI-TOF-MS方法对所选SNP位点进行基因分型

并应用对照病例遗传模型及单体型分析方法进行相关性研究.结果:PPARG基因所选的23个SNP位点具有多态性(MAF≥0.05).rs1801282、rs1899951、rs2881654、rs2972162位点基因型在对照和病例组中的分布除了在隐性模型中未见显著的统计学意义之外

在共显性模型和显性模型均存在显著的统计学意义(P<0.001

P<0.05)

rs2921190、rs2959272、rs1875796、rs1151999位点基因型在对照和病例组中的分布除了在共显性模型和隐性模型中未见显著的统计学意义之外

在显性模型存在显著的统计学意义(P<0.05).PPARG基因单体型区块Ⅰ7个SNPs(rs4684846

rs9817428

rs17036242

rs9310401

rs1801282

rs1899951

rs7615916)位点组成的4种主要单体型中GAACGAA单体型组间分布具有统计学差异(χ2=4.935

P=0.0263

P<0.05).单体型区块Ⅱ6个SNPs(rs2938397

rs2292101

rs2959273

rs4135275

rs709151

rs1175540)位点组成的6种主要的单体型组间分布均无统计学差异(P>0.05).结论:共显性、显性和隐性3个遗传模型下PPARG基因的rs1801282

rs1899951

rs2881654

rs2921190

rs2959272

rs1875796

rs4135275

rs1151999位点变异可能与柯尔克孜族人T2DM相关

GAACGAA单体型可能是柯尔克孜族T2DM的遗传标记.

Abstract

Objective: To study the relationship of PPARG gene haplotype with type 2 diabetes in Kirgiz people. Methods: Select 50 control and 50 patients with type 2 diabetes from Kirgiz people as control and case groups. Select 23 SNPs of PPARG gene(rs1151999

rs1175540

rs17036242

rs1875796

rs1899951

rs2292101

rs2881654

rs2921190

rs2938397

rs2959272

rs2959273

rs2972162

rs3856806

rs4135247

rs4135275

rs4684846

rs6782475

rs709151

rs7615916

rs7626560

rs9310401

rs9817428

rs1801282)

applied MALDITOF-MS-SNP genotyping methods for genotyping

and used case-control haplotypes analysis to study correlation. And studed corrilation through contrast with case genetic model and haplotype analysis method.Results: 23 SNP loci selected from PPARG gene have polymorphism(MAF = 0.05). Distribution of rs1801282

rs1899951

rs2881654

rs2972162 locus in case patients and control group showed no significant statistical significance in the recessive model

but in the codominant and dominant models there are statistical significance(P<0.001

P<0.05). Distribution of rs2921190

rs2959272

rs1875796

rs1151999 locus genotype in case patients and control group showed no statistical significance in the codominant and recessive model

but there are statistically significance in the dominant model(P<0.05). In four main haplotype's composed of seven SNPs(rs4684846

rs9817428

rs17036242

rs9310401 and rs1801282

rs1899951

rs7615916)site on PPARG gene haplotype block I

GAACGAA haplotype's distribution among groups have significant difference(χ2=4.935

P =0.0263

P<0.05).Distribution among groups in six main haplotype's composed of six SNPs(rs2938397

rs2292101

rs2959273

rs4135275

rs709151

rs1175540) site on haplotype block II

there are no significant difference(P<0.05). Conclusion: Under the three genetic models of codominant

dominant and recessive

PPARG gene's rs1801282

rs1899951

rs2881654

rs2921190

rs2959272

rs1875796

rs4135275

rs1151999 sites variation may be associated with Kirgiz T2 DM

and GAACGAA haplotype may the genetic markers of Kirgiz T2 DM.

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