油莎草实时荧光定量PCR内参基因的选择

敬思群; 于红; 郭改; 涂亦娴; 普燕

doi:10.13568/j.cnki.651094.2016.03.003

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油莎草实时荧光定量PCR内参基因的选择

更新时间：2026-01-21

- 油莎草实时荧光定量PCR内参基因的选择
- Journal of Xinjiang University (Natural Science Edition in Chinese and English) Vol. 33, Issue 3, Pages: 265-269(2016)
- 作者机构：
  
  新疆大学生命科学与技术学院
- 作者简介：
- 基金信息：
- DOI：10.13568/j.cnki.651094.2016.03.003
  CLC： S565.9;Q943.2
- Published：2016
- 稿件说明：
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[1]敬思群,于红,郭改,等.油莎草实时荧光定量PCR内参基因的选择[J].新疆大学学报(自然科学版),2016,33(03):265-269+379.
[1]敬思群,于红,郭改,等.油莎草实时荧光定量PCR内参基因的选择[J].新疆大学学报(自然科学版),2016,33(03):265-269+379. DOI： 10.13568/j.cnki.651094.2016.03.003.

DOI：10.13568/j.cnki.651094.2016.03.003.

摘要

目的筛选不同生长时期的油莎草地上茎的内参基因

为分析其基因的表达提供支持.方法以生长40 d的油莎草幼苗10:00

12:00

14:00

16:00

18:00

20:00六个时间点的地上茎为材料;以及生长天数为20

105

115

125

135

145 d的油莎草地上茎为材料

采用实时荧光定量PCR(Quantitative real-time PCR

RT-q PCR)技术

分析其18S r RNA

GAPDH-两个候选基因.结论筛选出18S r RNA作为分析油莎草地上茎不同时期基因表达量的内参基因.

Abstract

[Objective] The objective of this experiment was to select reference gene of different growth periods of stems of Cyperusesculentus. L to support analysis of the gene expression. [Method] In this study

quantitative real-time PCR(RT-q PCR) was used to analyze the m RNA expression of two candidate reference genes

18 S r RNA and GAPDH

in six time point(10:00

12:00

14:00

16:00

18:00

20:00) stems of Cyperusesculentus. L forty days and in stems of Cyperusesculentus.L seedlings grown different days(20

105

115

125

135

145 d). [Result] 18 SrRNA was selected to reference gene of analyzing the gene expression of different growth periods.

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