一株鲫鱼致病性镰刀菌的分离鉴定及其生物学特性

张艳珍; 付龙威; 王咏星; 刘云国

doi:10.13568/j.cnki.651094.651316.2019.12.23.0002

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一株鲫鱼致病性镰刀菌的分离鉴定及其生物学特性

更新时间：2026-01-21

- 一株鲫鱼致病性镰刀菌的分离鉴定及其生物学特性
- Journal of Xinjiang University (Natural Science Edition in Chinese and English) Vol. 38, Issue 1, Pages: 76-82(2021)
- 作者机构：
  
  1. 临沂大学生命科学学院
  2. 新疆大学生命科学与技术学院
- 作者简介：
- 基金信息：
- DOI：10.13568/j.cnki.651094.651316.2019.12.23.0002
  CLC： S943
- Published：2021
- 稿件说明：
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[1]张艳珍,付龙威,王咏星,等.一株鲫鱼致病性镰刀菌的分离鉴定及其生物学特性[J].新疆大学学报(自然科学版)(中英文),2021,38(01):76-82.
[1]张艳珍,付龙威,王咏星,等.一株鲫鱼致病性镰刀菌的分离鉴定及其生物学特性[J].新疆大学学报(自然科学版)(中英文),2021,38(01):76-82. DOI： 10.13568/j.cnki.651094.651316.2019.12.23.0002.

DOI：10.13568/j.cnki.651094.651316.2019.12.23.0002.

摘要

探究一株分离自新疆地区患病鲫鱼(Carassius auratus)病原菌的生物学特性.从患病鲫鱼体表分离纯化得到一株丝状真菌JC01

经人工感染鲫鱼卵试验验证JC01具有高致病性

研究其形态特征和生长特性

并进行ITS rDNA序列测定以及系统发育分析.菌株JC01在马铃薯葡萄糖琼脂培养基(PDA)上生长良好

菌落大且边缘整齐

结构干燥疏松

正面雪白背面呈黄色

菌丝为分支有隔菌丝

呈透明管状

具有镰刀形分生孢子和球状厚垣孢子

其最适生长温度、pH、盐度分别为28℃、7、3%.系统发育树显示菌株JC01与镰刀属菌株自然聚类

同源性高达99%

其中与Fusarium langsethiae(GenBank登录号MG274309.1)的亲缘关系最近

结合形态特征

将JC01鉴定为Fusarium langsethiae.本文新发现的一株丝状真菌为鲫鱼致病性镰刀菌

对其生物学特性进行的初步研究结果

可为鲫鱼镰刀菌病的防治提供理论依据.

Abstract

The aim was to explore the pathogenic bacteria from infected Crucian carp(Carassius auratus) in Xinjiang. A filamentous fungus JC01 was isolated from the surface of diseased Crucian carp(Carassius auratus).Artificial infection of Crucian carp eggs proved that JC01 was highly pathogenic. In this research

the morphological and growth characteristics were studied

ITS rDNA sequencing and phylogenetic analysis were performed of the strain. The strain JC01 grew well on potato glucose agar medium(PDA)

and its optimum temperature

p H and concentration of sodium chloride were 28 ℃

7 and 3% respectively. The main morphological characteristics are large colony with neat edge

dry and loose structure

yellow on the front and back of snow-white. The hyphae of strain JC01 were branched septate hyphae

transparent tubular

with falcate conidia and spherical chlamydospore.A phylogenetic tree was constructed

which showed that strain JC01 had 99% homology with Fusarium strains

and had the closest relationship with F usarium langsethiae. Therefore

JC01 was identified as F usarium langsethiae by morphological characteristics and ITS rDNA sequence analysis. In this paper

a new pathogenic F usarium sp.was isolated and identified from Crucian carp

and preliminary research results on its biological characteristics can provide theoretical basis for the prevention and treatment of Fusariumsis in Crucian carp.

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references

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