石油污染土壤自然老化过程中微生物菌群及功能基因变化规律

李兴海; 吕杰; 马媛; 吕光辉

doi:10.13568/j.cnki.651094.651316.2020.03.02.0001

您当前的位置：

首页 >

文章列表页 >

石油污染土壤自然老化过程中微生物菌群及功能基因变化规律

更新时间：2026-01-21

- 石油污染土壤自然老化过程中微生物菌群及功能基因变化规律
- Journal of Xinjiang University (Natural Science Edition in Chinese and English) Vol. 38, Issue 4, Pages: 472-480(2021)
- 作者机构：
  
  新疆大学资源与环境科学学院
- 作者简介：
- 基金信息：
- DOI：10.13568/j.cnki.651094.651316.2020.03.02.0001
  CLC： S154.3;X53
- Published：2021
- 稿件说明：
移动端阅览
[1]李兴海,吕杰,马媛,等.石油污染土壤自然老化过程中微生物菌群及功能基因变化规律[J].新疆大学学报(自然科学版)(中英文),2021,38(04):472-480.
[1]李兴海,吕杰,马媛,等.石油污染土壤自然老化过程中微生物菌群及功能基因变化规律[J].新疆大学学报(自然科学版)(中英文),2021,38(04):472-480. DOI： 10.13568/j.cnki.651094.651316.2020.03.02.0001.

DOI：10.13568/j.cnki.651094.651316.2020.03.02.0001.

摘要

本文旨在揭示新疆准东油田石油污染土壤自然老化过程中微生物菌群结构及芳香烃双加氧酶基因多态性及其变化规律.采集同一采油区3个不同污染年限土壤样本及背景土

进行理化指标检测、16S rDNA V4-V5区扩增子测序及芳香烃双加氧酶基因克隆文库构建与分析.扩增子测序分析结果显示

采油区不同背景土壤中原核微生物种类具有极大的相似性

并且污染1年的土壤中微生物种类与背景土微生物种类相似性较大;污染3年和5年样本间有一定差异

组内差异较小.克隆文库共获得46个芳香烃双加氧酶基因OTUs(Operational Taxonomic Units)

其Genbank注册号为KY985598-KY985643

分为邻苯二甲酸双加氧酶基因、非培养放线菌芳香加氧酶基因、萘双加氧酶基因、多环芳烃双加氧酶基因和苯甲酸双加氧酶基因5个基因类型

不同石油污染年限的土壤中双加氧酶基因类型会有显著性差异.对不同污染年限土壤中微生物与环境因子进行相关性分析

结果显示:随石油污染年限的延长

土壤微生物群落结构及丰度呈规律性变化.准东油田石油污染土壤当中部分种类微生物和功能基因可作为检测石油污染物老化程度的分子标准.

Abstract

This paper aims to investigate the change rule of bacteria and aromatic dioxygenase gene diversity in oil-contaminated soil with different years from Zhundong oilfield

Xinjiang

China. The oil-contaminated and background soils of three different pollution years were collected in the same oil exploring area

and the soil physical and chemical properties were detected. The V4-V5 hypervariable regions of bacterial 16 S rRNA gene were amplified and sequenced and the aromatic dioxygenase gene clone library were constructed with the different soil samples.The 16 S rRNA gene sequencing results showed that the bacterial community structure and diversity are similarity in different background soils and the bacterial diversity in the soil polluted for 1 year is similar with that in the background soils. There were some differences in bacterial community between the samples of 3 years and 5 years of pollution

but there was little difference within the group respectively. 46 OTUs of aromatic dioxygenase gene were obtained

and the GenBank accession numbers were KY985598-KY985643. After the homologous analysis

the aromatic dioxygenase gene library were divided into five groups

which were phthalate dioxygenase gene

uncultured actinobacterium aromatic oxygenase gene

naphthalene dioxygenase gene

PAH ring-hydroxylating dioxygenase gene and benzoate dioxygenase gene. There were significant differences in the dioxygenase gene types in soils with different oil pollution years. The correlation between microbial community with environmental factors of soils with different pollution years were analyzed. The results showed that the microbial community structure and abundance changed regularly with the increase of pollution years. The paper implied that some microbial species and functional genes can be served as a molecular standard to estimate the degree of aged oil sludge in contaminated soil of Zhundong oilfield.

关键词

Keywords

references

任磊,黄廷林.土壤的石油污染[J].农业环境保护,2000,19(6):360-363.REN L,HUANG T L.Contamination of petroleum[J].Agro-environmental Protection,2000,19(6):360-363.(in Chinese)

RAVELET C,GROSSET C,MONTUELLE B,et al.Liquid chromatography study of pyrene degradation by two micromycetes in a freshwater sediment[J].Chemosphere,2001,44(7):1541-1546.

朱林海,丁金枝,王健健,等.石油污染对土壤-植物系统的生态效应[J].应用与环境生物学报,2012,18(2):320-330.ZHU L H,DING J Z,WANG J J,et al.Ecological effects of oil pollution on soil-plant system[J].Chinese Journal of Applied and Environmental Biology,2012,18(2):320-330.(in Chinese)

刘薇薇,塔娜,赵星华,等.我国环境中多环芳烃的健康风险评价进展[J].环境与健康杂志,2014,31(12):1104-1108.LIU W W,TA N,ZHAO X H,et al.Research progress on health risk assessments of PAHs in China[J].Journal of Environment and Health,2014,31(12):1104-1108.(in Chinese)

LI H,BOUFADEL M C.Long-term persistence of oil from the Exxon Valdez spill in two-layer beaches[J].Nature Geoscience,2010,3(2):96-99.

FIELD J A,SIERRA-ALVAREZ R.Microbial transformation and degradation of polychlorinated biphenyls[J].Environmental Pollution,2008,155(1):1-12.

SHAHI A,AYDIN S,INCE B,et al.Evaluation of microbial population and functional genes during the bioremediation of petroleum-contaminated soil as an effective monitoring approach[J].Ecotoxicology and Environmental Safety,2016,125:153-160.

HAN X M,LIU Y R,ZHENG Y M,et al.Response of bacterial pdo1,nah,and C12O genes to aged soil PAH pollution in a coke factory area[J].Environmental Science and Pollution Research,2014,21:9754-9763.

于寒颖,杨慧.石油烃降解酶及其基因的结构、功能和表达调控[J].应用与环境生物学报,2012,18(6):1066-1074.YU H Y,YANG H.Structure,Function and expression regulation of hydrocarbon-degrading enzymes and their encoding genes[J].Chinese Journal of Applied and Environmental Biology,2012,18(6):1066-1074.(in Chinese)

许炳雯,李诗阳,张强,等.细菌芳烃外二醇双加氧酶研究进展[J].应用与环境生物学报,2012,18(5):873-879.XU B W,LI S Y,ZHANG Q,et al.Advance in research on bacterial aromatic extradiol dioxygenase[J].Chinese Journal of Applied and Environmental Biology,2012,18(5):873-879.(in Chinese)

GIBSON D T,PARALES R E.Aromatic hydrocarbon dioxygenases in environmental biotechnology[J].Current Opinion in Biotechnology,2000,11(3):236-243.

ABU-OMAR M M,LOAIZA A,HONTZEAS N.Reaction mechanisms of mononuclear non-heme iron oxygenases[J].Chemical Reviews,2005,105(6):2227-2252.

章俭,夏春谷.芳香烃双加氧酶的结构与功能研究[J].化学进展,2004,16(1):116-122.ZHANG J,XIA C G.Studies of structure and function of aromatic hydrocarbon dioxygenases[J].Progress in Chemistry,2004,16(1):116-122.(in Chinese)

LIANG Y T,ZHANG X,WANG J,et al.Spatial variations of hydrocarbon contamination and soil properties in oil exploring fields across China[J].Journal of Hazardous Materials,2012,241-242:371-378.

朱秉启,于静洁,秦晓光,等.新疆地区沙漠形成与演化的古环境证据[J].地理学报,2013,68(5):661-679.ZHU B Q,YU J J,QIN X G,et al.Formation an evolution of sandy deserts in Xinjiang:the paleo-environmental evidences[J].Acta Geographica Sinica,2013,68(5):661-679.(in Chinese)

陈伟胜,童玲,唐国斌.石油污染土壤含水率的测定[J].华北水利水电学院学报,2012,33(4):93-96.CHEN W S,TONG L,TANG G B.Determination of water content of the oil-contaminated soil[J].Journal of North China University of Water Resources and Electric Power,2012,33(4):93-96.(in Chinese)

鲁如坤.土壤农业化学分析方法[M].北京：中国农业科技出版社,2000.LU R K.Analytical methods for soil and agricultural chemistry[M].Beijing:China Agricultural Science Press,2000.(in Chinese)

孙玉萍,刘素辉,徐晓燕,等.新疆不同石油产区油污土壤含油量的测定[J].疾病预防控制通报,2011,26(2):8-10.SUN Y P,LIU S H,XU X Y,et al.Determination of mineral oil content in soil in different oildom of Xinjiang[J].Bulletin of Disease Control and Prevention,2011,26(2):8-10.(in Chinese)

QUINCE C,LANZEN A,DAVENPOR R J,et al.Removing noise from pyrosequenced amplicons[J].BMC Bioinformatics,2011,12:38.

MAGOˇC T,SALZBERG S L.Flash:fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics,2011,27(21):2957-2963.

CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.

HAAS N J,GEVERS D,EARL A M,et al.Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons[J].Genome Research,2011,21:494-504.

EDGAR R C.Uparse:highly accurate OTU sequences from microbial amplicon reads[J].Nature Methods,2013,10:996-998.

WANG Q,GARRITY G M,TIEDJE J M,et al.Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J].Applied and Environmental Microbiology,2007,73(16):5261-5267.

ZHOU H W,GUO C L,WONG Y S,et al.Genetic diversity of dioxygenase genes in polycyclic aromatic hydrocarbon-degrading bacteria isolated from mangrove sediments[J].FEMS Microbiology Letters,2006,262:148-157.

SUDHIR K,GLEN S,KOICHIRO T.MEGA7:Molecular evolutionary genetics analysis version 7.0 for bigger datasets[J].Molecular Biology and Evolution,2016,33(7):1870-1874.

杨智,陈吉祥,周永涛,等.玉门油田污染荒漠土壤石油降解菌多样性[J].环境科学研究,2017,30(5):799-808.YANG Z,CHEN J X,ZHOU Y T,et al.Diversity of oil-degrading bacteria isolated from oil-contaminated desert soil of Yumen oilfield[J].Research of Environmental Sciences,2017,30(5):799-808.(in Chinese)

蔡萍萍,宁卓,何泽,等.采油井场土壤微生物群落结构分布[J].环境科学,2018,38(7):3329-3338.CAI P P,NING Z,HE Z,et al.Microbial community distributions in soils of an oil exploitation site[J].Environmental Science,2018,38(7):3329-3338.(in Chinese)

谭银萍,马媛,吕杰.石油污染对土壤细菌结构特征的影响[J].生物技术,2016,26(2):193-198.TAN Y P,MA Y,LYU J.Effect of oil pollution on soil bacteria structure characteristics[J].Biotechnology,2016,26(2):193-198.(in Chinese)

IWAI S,JOHNSON T A,CHAI B,et al.Comparison of the specificities and effcacies of primers for aromatic dioxygenase gene analysis of environmental samples[J].Applied Environmental Microbiology,2011,77(11):3551-3557.

JIN D C,LIANG R X,DAI Q Y,et al.Biodegradation of di-n-butyl phthalate by Rhodococcus sp.JDC-11 and molecular detection of 3,4-phthalate dioxygenase gene[J].Journal of Microbiology and Biotechnology,2010,20(10):1440-1445.

SAITO A,IWABUCHI T,HARAYAMA S.A novel phenanthrene dioxygenase from Nocardioides sp.strain KP7:expression in Escherichia coli[J].Journal of Bacteriology,2000,182(8):2134-2141.

BACOSA H P,ERDNER D L,ROSENHEIM B E,et al.Hydrocarbon degradation and response of seafloor sediment bacterial community in the northern Gulf of Mexico to light Louisiana sweet crude oil[J].The ISME Journal,2018,12:2532-2543.

Views

516

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

低剂量辐射下土壤细菌群落及其功能基因的响应

Related Author

吴娜

杨多

赵鲁玉

岳海涛

Related Institution

新疆大学生命科学与技术学院

⁰