低剂量辐射下土壤细菌群落及其功能基因的响应

吴娜; 杨多; 赵鲁玉; 岳海涛

doi:10.13568/j.cnki.651094.651316.2020.10.23.0003

您当前的位置：

首页 >

文章列表页 >

低剂量辐射下土壤细菌群落及其功能基因的响应

更新时间：2026-01-21

- 低剂量辐射下土壤细菌群落及其功能基因的响应
- Journal of Xinjiang University (Natural Science Edition in Chinese and English) Vol. 38, Issue 5, Pages: 588-598(2021)
- 作者机构：
  
  新疆大学生命科学与技术学院
- 作者简介：
- 基金信息：
- DOI：10.13568/j.cnki.651094.651316.2020.10.23.0003
  CLC： S154.3
- Published：2021
- 稿件说明：
移动端阅览
[1]吴娜,杨多,赵鲁玉,等.低剂量辐射下土壤细菌群落及其功能基因的响应[J].新疆大学学报(自然科学版)(中英文),2021,38(05):588-598.
[1]吴娜,杨多,赵鲁玉,等.低剂量辐射下土壤细菌群落及其功能基因的响应[J].新疆大学学报(自然科学版)(中英文),2021,38(05):588-598. DOI： 10.13568/j.cnki.651094.651316.2020.10.23.0003.

DOI：10.13568/j.cnki.651094.651316.2020.10.23.0003.

摘要

为研究低剂量辐射对土壤微生物组成与丰度的影响

分析微生物组中对辐射胁迫的主效基因

本研究从新疆和布克赛尔蒙古自治县关闭回填的铀矿场采集了辐射剂量从7.71 m Sv/y到15.07 mSv/y不等的土壤样品.应用宏基因组测序结合生物信息学分析方法

发现低剂量辐射环境下的土壤中变形菌门、放线菌门、拟杆菌门、芽单胞菌门、绿弯菌门和酸杆菌亚门为优势菌门

其丰度总和占比达80%以上.属水平上

假单胞菌属数量最多

丰度最高

占31.0%;芽孢杆菌属次之

为11.0%;肠杆菌属为10.3%.辐射剂量高的土壤中

微生物OTU数量最高

多样性丰富度最大.土壤样品中微生物群落碳氮源的代谢强度也与辐射剂量呈正相关.宏基因组测序及分析结果表明:辐射土壤样品中微生物功能基因达到7 558个

比正常辐射水平对照组高11.3%

高辐射样品中碱基切除修复通路中的mug(TDG/mug DNA糖基化酶家族蛋白)的数量显著高于对照组和低辐射组.从辐射土壤样品中分离出30个属的可培养菌145株

其中一株藤黄微球菌Micrococcus luteus R17具有辐射和盐碱逆境的耐受性

可作为潜在的底盘菌株加以改造.

Abstract

To study the effects of low-dose radiation on soil microbial composition and abundance

and to analyze the primary genes in the microbiome that respond to radiation stress

soil samples with radiation doses ranging from 7.71 mSv/y to 15.07 mSv/y were collected from uranium mines in Xinjiang and Buccaneer County that were closed for reclamation in this study. Metagenomics Sequencing combined with bioinformatics analysis was applied

and it was found that the 6 phyla of Proteobacteria

Actinobacteria

Bacteroidetes

Gemmatimonadetes

Chloroflexi and Acidobacteria subphylum were the dominant in the soil under low dose radiation environment

with their combined abundance accounting for more than 80% of the total. At the genus level

Pseudomonasspp. is the most abundant and highest in abundance

accounting for 31.0%

followed by Bacillus spp. with 11.0% and Enterobacteriaceae with 10.3%. The highest number of microbial OTUs and the greatest diversity richness were found in soils with high radiation doses. The metabolic intensity of carbon and nitrogen sources of microbial communities in soil samples was also positively correlated with radiation dose. The number of mug(TDG/mug DNA glycosylase family proteins) in the base excision repair pathway was significantly higher in the irradiated soil samples than in the control and low irradiation groups. One strain of Micrococcus luteus R17

145 culturable strain of 30 genera

was isolated from the irradiated soil samples

which was resistant to radiation and saline-alkali stressand could be modified as a potential chassis strain.

关键词

Keywords

references

UNITED N.Sources,effects and risk of ionizing radiation[R].New York:Unscear Report,1988.

UNSCEAR.The United Nationsscientific committee on the effect of atomic radiation[J].Health Physics,2000,79(3):314.

Al ONSO-HEMANDEZ CM,TOLEDO-SIBELLOAL,GUILLEN-ARRUEBAREN A,et al.Natural radioactivity and evaluation of radiation hazards in soils from granitoide-granite geological formation in cuba[J].Rad Protect,2019,184:5-11.

ROQUIER C,REGENSPURG S,HARFOUVHE M,et al.Binding of uranium to organic-rich soils in an alpine region in Switzerland[J].Geochmica Et Cosmochimica Acta,2010,74:2082-2098.

BAI A Q,FU B J,QU L Y,et al.The characteristics of soil microbial communities atburned forest sites for the Great Xingan mountains[J].Acta Ecologica Sinica,2012,32:4762-4771.

BURRELL A,DFELDSCHREIBER P,DEAN C J.DNA-membrane association and the repair of double breaks in X-irradiated Micrococcus radiodurans[J].Biochim Biophys Acta,1971,247:38-53.

BONUR A K,SMITH K C.The involvement of indirect effects in cell-killing and DNA double-strand breakage inγ-irradiated Escherichia coli K-12[J].Int J Rad Biol,1976,29:293-296.

BEBLO K,RABBOW E,RACHEL R,et al.Tolerance of thermophilic and hyperthermophilic microorganisms to desiccation[J].Extremophile,2009,13:521-531.

BEBLO K,RABBOW E,RACHEL R,et al.Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C,ionizing radiation and desiccation[J].Arch Microbiol,2011,193:797-809.

KUJAWA J,ZAVODNIK L,ZAVODNIK I,et al.Effect of low-intensity (3.75-25 J/cm2) near-infrared (810nm) laser radiation on red blood cell ATPase activities and membrane structure[J].J Clin Laser Med Surg,2004,22:111-117.

CABISCOL E,TAMARIT J,ROS J.Oxidative stress in bacteria and protein damage by reactive oxygen species[J].Int Microbiol,2000,3:3-8.

ROTHKAMMK,KRUGER I,THOMPSON L H,et al.Pathways of DNA double-strand break repair during the mammalian cell cycle[J].Mol Cell Biol,2003,23:5706-5715.

KOONIN E V,WOIF Y I.Genomics of bacteria and archaea:the emerging dynamic view of the prokaryotic world[J].Nucleic Acids Res,2008,36:6688-6719.

DANIEL R.The metagenomics of soil[J].Nat Rev Microbiol,2005,3:470-478.

RENSHAW J C,LLOYD J R,LIVENS F R.Microbial interactions with actinides and long-lived fission products[J].CR-Chimie,2007,10:1067-1077.

LANDA E R.Uranium mill tailings:nuclear waste and natural laboratory for geochemical and radioecological investigations[J].J Environ Radioact,2004,77:1-27.

ZHDANOVA N N,ZAKHARCHENKO V A,VEMBER V V,et al.Fungi from Chernobyl:mycobiota of the inner regions of the containment structures of the damaged nuclear reactor[J].Mycol Res,2000,104:1421-1426.

SHUKLA A,PARMAR P,SARAF M.Radiation,radionuclides and bacteria:An in-perspective review[J].J Environ Radioact,2017,180:27-35.

GAZSO L G.The key microbial processes in the removal of toxic metals and radionuclides from the environment[J].Central Eur J Occup Environ Med,2001,7:178-185.

ABDELOUAS A,LU Y M,LUTZW W.Reduction of U (Ⅳ) to U (Ⅳ) by indigenous bacteria in contaminated ground water[J].J Contam Hydrol,1998,35:217-233.

QUAST C,PRUESSE E,YILMAZP,et al.The SILVA ribosomal RNA gene database project:improved data processing and web-based tools[J].Nuc Ac Res,2013,41:D590-6.

MAGOCET T,SALZBERG S L.FLASH:fast length adjustment of short reads to improve genome assemblies[J].Bioinform,2011,27:2957-2963.

BOLGERA M,LOHSE M,USADELB.Trimmomatic:aflexible trimmer for Illumina sequence data[J].Bioinform,2014,30:2114-20.

EDGAR R C,HAAS B J,CLEMENTE J C,et al.UCHIME improves sensitivity and speed of chimera detection[J].Bioinform,2011,27:2194-2200.

BOKULICH N A,SUBRAMANIAN S,FAITHJ J,et al.Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing[J].Nat Meth,2013,10:57-59.

SCHLOSS P D,WESTCOTT S L,RYABIN T,et al.Introducing mothur:open-source,platform-independent,communitysupported software for describing and comparing microbial communities[J].App and Envir Microbio,2009,75:7537-7541.

EDGAR R C.Search and clustering orders of magnitude faster than BLAST[J].Bioinform,2010,26:2460-2461.

CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nat Meth,2010,7:335-336.

CHOIK H,DOBBSF C.Comparison of two kinds of Biolog microplates (GN and ECO) in their ability todistinguish among aquatic microbial communities[J].J of Microbio Meth,1999,36:203-213.

WANG P,SCHELLHOM H E.Induction of resistance to hydrogen peroxide and radiation in Deinococcus radiodurans[J].C J of Microbio,1995,41:170-176.

WU Y D,YANG Y,PENG G,et al.Radiation-Resistant Micrococcus luteus SC1204 and its proteomics change upon gamma irradiation[J].Curr Microbiol,2016,72:767-775.

NI S P,JOHN N,MAMATH C,et al.Characterization of bioactive compound produced by microfoulingactinobacteria (Micrococcus Luteus) isolated from the ship hull in Arabian Sea,Cochin.Kerala[J].Materials Today:Proceedings,2020,25:257-264.

ZHANG Z D,MAO J,TANG Q Y,et al.Diversity investigation of actinomycetes isolatedfrom radiation-polluted soil[J].Microbiology,2009,36:1329-1333.

GU M Y,ZHANG Z D,WANG W,et al.The effects of radiation pollution on the population diversities and metabolic characteristics of soil microorganisms[J].Water Air Soil Pollut,2014,225:2133.

GALLINAR I P,JIRICNY J.A new class of uracil-DNA glycosylases related to human thymine-DNA glycosylase[J].Nature,1996,383:735-738.

LIU P,BURDZY A,SOWERS L C.Substrate recognition by a family of Uracil-DNA glycosylases UNG,MUG,and TDG[J].Chemical Res in Toxico,2002,15:1001-1009.

DANIEL C,CHRISTOPHE K,YUSUKE S,et al.The enigmatic thymine DNA glycosylase[J].DNA Repair,2007,6:489-504.

Views

272

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

石油污染土壤自然老化过程中微生物菌群及功能基因变化规律

Related Author

李兴海

吕杰

马媛

吕光辉

Related Institution

新疆大学资源与环境科学学院

⁰