早春短生植物鸢尾蒜在干热和湿润环境下的比较转录组研究

李岩; 李周康; 张雪妮; 吕光辉

doi:10.13568/j.cnki.651094.651316.2020.12.29.0002

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早春短生植物鸢尾蒜在干热和湿润环境下的比较转录组研究

更新时间：2026-01-21

- 早春短生植物鸢尾蒜在干热和湿润环境下的比较转录组研究
- Journal of Xinjiang University (Natural Science Edition in Chinese and English) Vol. 39, Issue 1, Pages: 49-58(2022)
- 作者机构：
  
  新疆大学资源与环境科学学院绿洲生态教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13568/j.cnki.651094.651316.2020.12.29.0002
  CLC： Q943
- Published：2022
- 稿件说明：
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[1]李岩,李周康,张雪妮,等.早春短生植物鸢尾蒜在干热和湿润环境下的比较转录组研究[J].新疆大学学报(自然科学版)(中英文),2022,39(01):49-58.
[1]李岩,李周康,张雪妮,等.早春短生植物鸢尾蒜在干热和湿润环境下的比较转录组研究[J].新疆大学学报(自然科学版)(中英文),2022,39(01):49-58. DOI： 10.13568/j.cnki.651094.651316.2020.12.29.0002.

DOI：10.13568/j.cnki.651094.651316.2020.12.29.0002.

摘要

本研究对新疆干旱区早春短生植物鸢尾蒜进行转录组测序

通过比较转录组分析

揭示干热生境和林下湿润生境中鸢尾蒜个体基因表达的差异

探讨鸢尾蒜适应新疆干热环境的分子机理.转录组测序共得到150 702个同基因

检测到两种生境间显著差异表达基因1 929个

其中827个上调

1 102个下调.差异基因共富集到49个GO条目

下调基因功能主要包括代谢过程、生物合成、生物调节、细胞、细胞器、催化活性、结合活性等

上调基因功能包括繁殖、繁殖过程、信号、转导活性等. KEGG代谢通路富集发现下调基因显著富集到光合作用、碳代谢、氨基酸代谢和蛋白质加工等通路中

而上调基因显著富集到与细胞渗透压调节、抗氧化活性相关的次生代谢产物合成代谢及信号转导通路中.干热环境可能通过降低光合作用抑制鸢尾蒜的生长

植株通过激活信号转导、提高渗透调节物质和抗氧化活性以维持细胞的内稳态和正常功能;此外

发育过程和繁殖过程的促进保障了个体在干热环境中快速完成生活周期.研究结果为揭示新疆短生植物适应干热环境的分子调控机制以及适应性进化提供了基础.

Abstract

Transcriptome sequencing was performed on individuals of spring ephemeral plant Ixiolirion tataricum from two different habitats: dry and hot habitat and under canopy humid habitat in arid Xinjiang. We investigated the difference of gene expression between the two habitats by comparative transcriptomic analysis

aiming to discuss the adaptive mechanism of I. tataricum to the hot and dry environment in Xinjiang. A total of 150 702 unigenes were assembled from the raw reads. A total of 1 929 significantly differential expressed genes(DEGs) were detected between the two habitats

of which 827 were up-regulated and 1 102 down-regulated. DEGs were enriched into 49 gene ontology(GO) items. Down-regulated gene were mainly enriched in function groups metabolic processes

biosynthesis

biological regulation

cells

organelles

catalytic activity

binding activity

etc.

while up-regulated genes in reproduction

reproduction process

signal

transduction activity

etc.. KEGG metabolic pathway enrichment found that down-regulated genes were significantly enriched in photosynthesis

carbon metabolism

amino acid metabolism and protein processing

while up-regulated genes in secondary metabolites synthesis related to cell osmotic pressure regulation and antioxidant activity

and signal transduction pathways. The dry and hot environment inhibited the growth of I. tataricum by reducing photosynthesis and related biological processes. I. tataricum maintained the homeostasis and normal functions of cells by activating signal transduction

increasing osmotic regulation substances and antioxidant activities. In addition

it was enabled that I. tataricum complete the life cycle timely by promotion of the development process and reproduction process in dry and hot environment. This study will provide basic knowledge for revealing the molecular regulation and adaptive evolution of ephemeral plants in dry and hot environment.

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