HepG2细胞胰岛素抵抗模型建立及在筛选天然有效多糖中的应用

姜慰; 史茜; 刘晓颖; 付玉兰; 李金耀; 王为兰

doi:10.13568/j.cnki.651094.651316.2022.12.22.0001

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HepG2细胞胰岛素抵抗模型建立及在筛选天然有效多糖中的应用

更新时间：2026-01-21

- HepG2细胞胰岛素抵抗模型建立及在筛选天然有效多糖中的应用
- Journal of Xinjiang University (Natural Science Edition in Chinese and English) Vol. 40, Issue 4, Pages: 461-466(2023)
- 作者机构：
  
  1. 新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室
  2. 新疆生产建设兵团医院检验科
- 作者简介：
- 基金信息：
- DOI：10.13568/j.cnki.651094.651316.2022.12.22.0001
  CLC： R965.1
- Published：2023
- 稿件说明：
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[1]姜慰,史茜,刘晓颖,等.HepG2细胞胰岛素抵抗模型建立及在筛选天然有效多糖中的应用[J].新疆大学学报(自然科学版)(中英文),2023,40(04):461-466.
[1]姜慰,史茜,刘晓颖,等.HepG2细胞胰岛素抵抗模型建立及在筛选天然有效多糖中的应用[J].新疆大学学报(自然科学版)(中英文),2023,40(04):461-466. DOI： 10.13568/j.cnki.651094.651316.2022.12.22.0001.

DOI：10.13568/j.cnki.651094.651316.2022.12.22.0001.

摘要

探讨高糖、地塞米松、胰岛素诱导肝细胞HepG2胰岛素抵抗模型（IR-HepG2）的建立方法，检测杏鲍菇多糖、红枣多糖和枸杞多糖体外改善肝细胞胰岛素抵抗的作用，评估3种多糖的降糖效果．选择不同浓度的地塞米松、胰岛素及两者联合作用，在高糖培养条件下诱导HepG2胰岛素抵抗，MTT法检测不同诱导剂对HepG2细胞活性的影响，葡萄糖氧化酶法测定细胞葡萄糖消耗量，同时检测去除诱导剂后，IR-HepG2细胞模型的稳定性．以3种天然多糖处理IR-HepG2细胞，检测其对细胞活力及胰岛素抵抗的影响．结果表明：在高糖培养条件下，诱导剂为地塞米松（3.75μmol/L）联合胰岛素（0.001μmol/L）可成功诱导IR-HepG2细胞模型，且对细胞活性无显著影响，模型能够在72 h内保持稳定．枸杞多糖具有更好的降糖效果．

Abstract

To explore the establishment of insulin resistance model of HepG2(IR-HepG2) induced by high glucose

dexamethasone and insulin

to detect the effect of Pleurotus eryngii polysaccharides

Ziziphus jujuba polysaccharides and Lycium barbarum polysaccharides on improving insulin resistance of liver cells in vitro

and to analyze the effect of three polysaccharides on hypoglycemic. Different concentrations of dexamethasone

insulin and their combination were selected to induce the insulin resistance of HepG2 cells in high glucose culture. MTT method was used to detect the effect of different inducers on the activity of HepG2 cells. Glucose oxidase method was used to detect the glucose content in the supernatant of cell culture. At the same time

the stability of IR-HepG2 cell model was detected after removing inducers. IR-HepG2 cells were treated with three kinds of natural polysaccharides at different concentrations. The effects of polysaccharides on cell viability and insulin resistance were detected by MTT and glucose oxidase methods respectively. Dexamethasone(3.75 μmol/L) combined with insulin(0.001μmol/L) can successfully induce IR-HepG2 cell model in high glucose culture condition

and has no significant effect on cell activity

and the model can keep stable within 72 hours. Lycium barbarum polysaccharides had better hypoglycemic effects in vitro.

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