丙泊酚通过miR-21/PTEN通路抑制结肠癌HCT116细胞增殖

袁婷婷; 阿迪莱·库热西; 闫睿

doi:10.13568/j.cnki.651094.651316.2025.04.20.0002

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丙泊酚通过miR-21/PTEN通路抑制结肠癌HCT116细胞增殖

更新时间：2026-01-21

- 丙泊酚通过miR-21/PTEN通路抑制结肠癌HCT116细胞增殖
- Journal of Xinjiang University (Natural Science Edition in Chinese and English) Vol. 42, Issue 5, Pages: 632-640(2025)
- 作者机构：
  
  新疆医科大学附属肿瘤医院麻醉科
- 作者简介：
- 基金信息：
- DOI：10.13568/j.cnki.651094.651316.2025.04.20.0002
  CLC： R735.35;R614
- Published：2025
- 稿件说明：
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[1]袁婷婷,阿迪莱·库热西,闫睿.丙泊酚通过miR-21/PTEN通路抑制结肠癌HCT116细胞增殖[J].新疆大学学报(自然科学版中英文),2025,42(05):632-640.
[1]袁婷婷,阿迪莱·库热西,闫睿.丙泊酚通过miR-21/PTEN通路抑制结肠癌HCT116细胞增殖[J].新疆大学学报(自然科学版中英文),2025,42(05):632-640. DOI： 10.13568/j.cnki.651094.651316.2025.04.20.0002.

DOI：10.13568/j.cnki.651094.651316.2025.04.20.0002.

摘要

结肠癌作为全球高发的恶性肿瘤，其发病率和死亡率均位居癌症前列．麻醉药物丙泊酚在多种癌症中表现出抗肿瘤活性，其作用机制可能与调节miR-21的表达相关．本文重点关注丙泊酚通过调控miR-21-5p/PTEN信号通路对结肠癌细胞增殖和凋亡的影响机制，为结肠癌治疗提供新的潜在靶点．将HCT116细胞分为对照组、丙泊酚组、miR-21-5p NC组、miR-21-5p mimic组、丙泊酚+miR-21-5p NC组、丙泊酚+miR-21-5p mimic组，将miR-21-5p mimic或miR-21-5p NC转染至HCT116细胞后，使用CCK-8法检测细胞增殖，流式细胞仪检测细胞周期，qRT-PCR检测细胞中miR-21-5p和PTEN的基因表达，Western Blot(WB)检测PTEN、p-AKT等关键蛋白的表达，双荧光素酶基因报告实验检测miR-21-5p与PTEN的靶向关系．实验结果显示：与对照组相比，丙泊酚处理显著抑制HCT116细胞增殖．在分子机制方面，丙泊酚组miR-21-5p mRNA表达水平较对照组显著下调，同时伴随PTEN mRNA表达水平增加（P<0.05）.WB分析表明：miR-21-5p过表达可抑制PTEN蛋白表达并激活p-AKT信号，而丙泊酚处理能够逆转这一效应．双荧光素酶报告实验进一步证实，PTEN是miR-21-5p的靶基因．故丙泊酚可能通过抑制miR-21-5p，解除其对PTEN的靶向调控作用，从而影响细胞增殖．

Abstract

As a highly prevalent malignant tumor worldwide

colon cancer ranks among the top cancers in terms of morbidity and mortality.The anesthetic drug propofol exhibits antitumor activity in a variety of cancers

and its mechanism of action may be related to the regulation of miR-21 expression.The paper focuses on the effects of propofol on colon cancer cell proliferation and apoptosis by modulating the miR-21-5p/PTEN signaling pathway

aiming to reveal this molecular mechanism to provide new potential targets for colon cancer therapy.HCT116 cells are divided into control group

propofol group

miR-21-5p NC group

miR-21-5p mimic group

propofol+miR-21-5p NC group

propofol+miR-21-5p mimic group

and miR-21-5p mimic or miR-21-5p NC are transfected into HCT116cells

and CCK-8 method is used to detect cell proliferation

flow cytometry is used to detect cell cycle.qRT-PCR is used to detect gene expression of miR-21-5p and PTEN in cells.Western Blot(WB) is used to detect the expression of key proteins such as PTEN and p-AKT.A dual luciferase gene reporter assay is used to detect the targeting relationship between miR-21-5p and PTEN.The experimental results show that propofol treatment significantly inhibites the proliferation of HCT116 cells compared with the control group.In terms of molecular mechanisms

miR-21-5p mRNA expression level is significantly down-regulated in the propofol group compared with the control group

accompanied by an increase in PTEN mRNA expression level(P<0.05).WB analysis shows that miR-21-5p overexpression inhibites PTEN protein expression and activates p-AKT signaling

and propofol treatment is able to reverse this effect.Dual luciferase reporter assay further confirms that PTEN is a target gene of miR-21-5p.It is suggested that propofol may affect cell proliferation by inhibiting miR-21-5p and deregulating its targeted regulation of PTEN.

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references

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